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. 2015 Nov 25;10(11):e0143318.
doi: 10.1371/journal.pone.0143318. eCollection 2015.

Protective Effects of Lactobacillus plantarum CCFM8246 against Copper Toxicity in Mice

Fengwei Tian  1 , Yue Xiao  1 , Xiaoxiao Li  1 , Qixiao Zhai  1 , Gang Wang  1 , Qiuxiang Zhang  1 , Hao Zhang  1 , Wei Chen  1   2
Affiliations

Protective Effects of Lactobacillus plantarum CCFM8246 against Copper Toxicity in Mice

Fengwei Tian et al. PLoS One. .

Abstract

Lactobacillus plantarum CCFM8246, which has a relatively strong copper binding capacity and tolerance to copper ions, was obtained by screening from 16 lactic acid bacteria in vitro. The selected strain was then applied to a mouse model to evaluate its protective function against copper intoxication in vivo. The experimental mice were divided into an intervention group and a therapy group; mice in the intervention group received co-administration of CCFM8246 and a copper ion solution by gavage, while mice in the therapy group were treated with CCFM8246 after 4 weeks of copper exposure. In both two groups, mice treated with copper alone and that treated with neither CCFM8246 nor copper served as positive and negative controls, respectively. At the end of the experimental period, the copper content in feces and tissues, the activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum, and oxidation stress indices in liver and kidney tissue were determined. Learning and memory ability was evaluated by Morris water maze experiments. The results indicated that treatment with CCFM8246 significantly increased the copper content in feces to promote copper excretion, reduce the accumulation of copper in tissues, reverse oxidative stress induced by copper exposure, recover the ALT and AST in serum and improve the spatial memory of mice.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Copper binding capacity of bacterial strains.
The average of three replicates and standard deviation are presented. CCFM8246, CCFM8614 and CCFM8610 are Lactobacillus plantarum. CCFM38 is Lactobacillus casei. CCFM24 is Lactobacillus bulgaricus. CCFMLa is Lactobacillus acidophilus. CCFM32 is Lactobacillus thermophilus. CCFM39 and CCFM16 are Bifidobacterium. LGG is L. rhamnosus GG. CCFMLR and CCFM13 are Lactobacillus reuteri. CCFM14 is Lactobacillus sake. CCFML.hel is Lactobacillus helveticus. CCFM2-3 is Lactobacillus delbrueckii. CCFMZ7 is Lactobacillus fermentum.
Fig 2
Fig 2. The first kind of growth of strains in different copper ion MRS broths.
The subgraphs (A) to (E) show the tolerance ability of CCFM38, CCFM24, CCFMLa, CCFM39 and CCFM32, respectively.
Fig 3
Fig 3. The second kind of growth of strains in different copper ion MRS broths.
The subgraphs (A) to (H) show the tolerance ability of CCFM8246, CCFM8614, Bifidobacterium, CCFM8610, CCFMLR, CCFM13, CCFM14 and CCFM2-3, respectively.
Fig 4
Fig 4. The third kind of growth of strains in different copper ion MRS broths.
The subgraphs (A) to (C) show the tolerance ability of CCFML.hel, CCFMZ7 and LGG, respectively.
Fig 5
Fig 5. Copper content in wet feces of mice in intervention groups.
Values are mean ± SEM with 10 mice in each group. The letters a, b, c, d and e mean that in each period, groups with different letters differ significantly (p <0.05).
Fig 6
Fig 6. Copper content in wet feces of mice in therapy groups.
Values are mean ± SEM with 10 mice in each group. The letters a, b and c mean that in each period, groups with different letters differ significantly (p <0.05).
Fig 7
Fig 7. Copper content in livers, kidneys and brains of mice.
Values are mean ± SEM with 10 mice in each group. The letters a, b, c, d and e mean that in each tissue, groups with different letters differ significantly (p <0.05).
Fig 8
Fig 8. The activity of ALT and AST in serum of mice.
The letters a, b, c, d, e and f mean that for each index, groups with different letters differ significantly (p <0.05).
See this image and copyright information in PMC

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