Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016:1385:201-22.
doi: 10.1007/978-1-4939-3289-4_15.

Molecular Analyses of Transgenic Plants

Kurniawan Rudi Trijatmiko  1 , Felichi Mae Arines  1 , Norman Oliva  1 , Inez Hortense Slamet-Loedin  1 , Ajay Kohli  2
Affiliations

Molecular Analyses of Transgenic Plants

Kurniawan Rudi Trijatmiko et al. Methods Mol Biol. 2016.

Abstract

One of the major challenges in plant molecular biology is to generate transgenic plants that express transgenes stably over generations. Here, we describe some routine methods to study transgene locus structure and to analyze transgene expression in plants: Southern hybridization using DIG chemiluminescent technology for characterization of transgenic locus, SYBR Green-based real-time RT-PCR to measure transgene transcript level, and protein immunoblot analysis to evaluate accumulation and stability of transgenic protein product in the target tissue.

Keywords: Chemiluminescent; Digoxigenin; Gene expression; SYBR Green; Southern blot; Transformation; Transgene; Western blot; qRT-PCR.

PubMed Disclaimer

Publication types

LinkOut - more resources