Use of the isocitrate dehydrogenase structural gene for attachment of e14 in Escherichia coli K-12
- PMID: 2661545
- PMCID: PMC210168
- DOI: 10.1128/jb.171.7.4083-4084.1989
Use of the isocitrate dehydrogenase structural gene for attachment of e14 in Escherichia coli K-12
Abstract
The e14 element appears to be integrated into the Escherichia coli K-12 isocitrate dehydrogenase structural gene (icd). In being integrated, it replaced the last 52 codons of the gene with a closely related sequence. The two versions of the icd gene produce proteins of the same length but differ by 12 base substitutions that would cause two conservative amino acid replacements.
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