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. 2016 Jan 1;21(1):e30-8.
doi: 10.4317/medoral.20593.

TlR expression profile of human gingival margin-derived stem progenitor cells

Karim Fawzy-El-Sayed  1 , Mohamed Mekhemar, Sabine Adam-Klages, Dietrich Kabelitz, Christof Dörfer
Affiliations

TlR expression profile of human gingival margin-derived stem progenitor cells

Karim Fawzy-El-Sayed et al. Med Oral Patol Oral Cir Bucal. .

Abstract

Background: Gingival margin-derived stem/progenitor cells (G-MSCs) show remarkable periodontal regenerative potential in vivo. During regeneration, G-MSCs may interact with their inflammatory environment via toll-like-receptors (TLRs). The present study aimed to depict the G-MSCs TLRs expression profile.

Material and methods: Cells were isolated from free gingival margins, STRO-1-immunomagnetically sorted and seeded to obtain single colony forming units (CFUs). G-MSCs were characterized for CD14, CD34, CD45, CD73, CD90, CD105, CD146 and STRO-1 expression, and for multilineage differentiation potential. Following G-MSCs' incubation in basic or inflammatory medium (IL-1β, IFN-γ, IFN-α, TNF-α) a TLR expression profile was generated.

Results: G-MSCs showed all stem/progenitor cells' characteristics. In basic medium G-MSCs expressed TLRs 1, 2, 3, 4, 5, 6, 7, and 10. The inflammatory medium significantly up-regulated TLRs 1, 2, 4, 5, 7 and 10 and diminished TLR 6 (p≤0.05, Wilcoxon-Signed-Ranks-Test).

Conclusions: The current study describes for the first time the distinctive TLRs expression profile of G-MSCs under uninflamed and inflamed conditions.

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Conflict of interest statement

Conflict of interest statement: The authors have declared that no conflict of interest exist.

Figures

Figure 1
Figure 1
Microscopic appearance, colony-formation, surface marker expression and differentiation potential: (A) Phase contrast microscopic appearance of the adherent tissue mass with outgrowing cells (2nd week). (B) Colony-forming units of G-MSCs (crystal violet). (C) Flow cytometric analysis of the surface marker expression profile of G-MSCs. Multilineage differentiation potential of G-MSCs. Alizarin Red staining of the osteogenically stimulated G-MSCs (D) and their controls (E) Oil-Red-O staining of the adipogenically stimulated G-MSCs (F) and their controls (G). Alcian Blue staining of the chondrogenically stimulated G-MSCs (H) and their controls (I).
Figure 2
Figure 2
Median Flouresence Intensity (MFI) of expressed TLRs in G-MSC and G-MSC-i: (A) Median Fluorescence Intensity (MFI) of expressed TLRs 1-10 of G-MSCs (green curve) and of their isotype controls (red curve) after incubation in basic medium. (B) Protein expression of TLRs 1-10 (n=5;box- and whisker plots with medians and quartiles). (C) Median Fluorescence Intensity (MFI) of expressed TLRs 1-10 of G-MSCs (green curve) and of their isotype controls (red curve) after incubation in inflammatory medium (G-MSCs-i). (D) Protein expression of TLRs 1-10 after incubation in inflammatory medium (n=5; box- and whisker plots with medians and quartiles). The green colored boxes show increased MFI after stimulation by the inflammatory medium (Wilcoxon Signed Rank test, statistical significance marked with asterisk, *:p<0.05).
Figure 3
Figure 3
Differences in mRNA expression of TLRs 1-10 in G-MSCs and G-MSC-i: (A) m-RNA expression of TLRs 1-10 in uninflamed condition. (B) m-RNA expression of TLRs 1 to 10 in inflamed condition (n=5; box- and whisker plots with medians and quartiles). The green colored boxes show increased mRNA expression after stimulation by the inflammatory medium (Wilcoxon Signed Rank test, statistical significance marked with asterisk, *:p<0.05).
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References

    1. Larjava H, Wiebe C, Gallant-Behm C, Hart DA, Heino J, Häkkinen L. Exploring scarless healing of oral soft tissues. J Can Dent Assoc. 2011;77:b18. - PubMed
    1. Pitaru S, McCulloch CA, Narayanan SA. Cellular origins and differentiation control mechanisms during periodontal development and wound healing. J Periodontal Res. 1994;29:81–94. - PubMed
    1. Sempowski GD, Borrello MA, Blieden TM, Barth RK, Phipps RP. Fibroblast heterogeneity in the healing wound. Wound Repair Regen. 1995;3:120–31. - PubMed
    1. Schor SL, Ellis I, Irwin CR, Banyard J, Seneviratne K, Dolman C. Subpopulations of fetal-like gingival fibroblasts: characterisation and potential significance for wound healing and the progression of periodontal disease. Oral Dis. 1996;2:155–66. - PubMed
    1. Phipps RP, Borrello MA, Blieden TM. Fibroblast heterogeneity in the periodontium and other tissues. J Periodontal Res. 1997;32:159–65. - PubMed

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