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. 2015 Sep 1;8(9):10139-47.
eCollection 2015.

Baicalin ameliorates isoproterenol-induced acute myocardial infarction through iNOS, inflammation and oxidative stress in rat

Huaguo Chen  1 , Yongfu Xu  1 , Jianzhong Wang  1 , Wei Zhao  1 , Huihui Ruan  1
Affiliations

Baicalin ameliorates isoproterenol-induced acute myocardial infarction through iNOS, inflammation and oxidative stress in rat

Huaguo Chen et al. Int J Clin Exp Pathol. .

Abstract

Baicalin belongs to glucuronic acid glycosides and after hydrolysis baicalein and glucuronic acid come into being. It has such effects as clearing heat and removing toxicity, anti-inflammation, choleresis, bringing high blood pressure down, diuresis, anti-allergic reaction and so on. In this study, we investigated whether baicalin ameliorates isoproterenol-induced acute myocardial infarction and its mechanism. Rat model of acute myocardial infarction was induced by isoproterenol. Casein kinase (CK), the MB isoenzyme of creatine kinase (CK-MB), lactate dehydrogenase (LDH), cardiac troponin T (cTnT) and infarct size measurement were used to measure the protective effect of baicalin on isoproterenol-induced acute myocardial infarction. iNOS protein expression in rat was analyzed using western blot analysis. Tumor necrosis factor-alpha (TNF-α), interleukin 6 (IL-6), malondialdehyde (MDA) and superoxide dismutase (SOD) and caspase-3 activation levels were explored using commercial ELISA kits. In the acute myocardial infarction experiment, baicalin effectively ameliorates the level of CK, CK-MB, LDH and cTnT, reduced infarct size in acute myocardial infarction rat model. Meanwhile, treatment with baicalin effectively decreased the iNOS protein expression, inflammatory factors and oxidative stresses in a rat model of acute myocardial infarction. However, baicalin emerged that anti-apoptosis activity and suppressed the activation of caspase-3 in a rat model of acute myocardial infarction. The data suggest that the protective effect of baicalin ameliorates isoproterenol-induced acute myocardial infarction through iNOS, inflammation and oxidative stress in rat.

Keywords: Baicalin; INOS; acute myocardial infarction; inflammation; isoproterenol; oxidative stress.

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Figures

Figure 1
Figure 1
The chemical structure of baicalin.
Figure 2
Figure 2
Baicalin ameliorates infarct size in acute myocardial infarction rat model. Control, control group; Isoflurane, isoflurane group; Low-dose, isoflurane + low-dose baicalin group (1 mg/mg); Medium-dose, isoflurane + medium-dose baicalin group (10 mg/mg); High-dose, isoflurane + high-dose baicalin group (100 mg/mg). #P < 0.01 compared with isoflurane group.
Figure 3
Figure 3
Baicalin ameliorates iNOS protein expression in a rat model of acute myocardial infarction. Baicalin ameliorates iNOS protein expression (A) and statistical analysis of iNOS protein expression (B) in a rat model of acute myocardial infarction. Control, control group; Isoflurane, isoflurane group; Low-dose, isoflurane + low-dose baicalin group (1 mg/mg); Medium-dose, isoflurane + medium-dose baicalin group (10 mg/mg); High-dose, isoflurane + high-dose baicalin group (100 mg/mg). #P < 0.01 compared with isoflurane group.
Figure 4
Figure 4
Baicalin ameliorates inflammation in a rat model of acute myocardial infarction. Baicalin ameliorates the TNF-α and IL-6 levels in a rat model of acute myocardial infarction. Control, control group; Isoflurane, isoflurane group; Low-dose, isoflurane + low-dose baicalin group (1 mg/mg); Medium-dose, isoflurane + medium-dose baicalin group (10 mg/mg); High-dose, isoflurane + high-dose baicalin group (100 mg/mg). #P < 0.01 compared with isoflurane group.
Figure 5
Figure 5
Baicalin ameliorates oxidative stress in a rat model of acute myocardial infarction. Baicalin ameliorates the MDA and SOD levels in a rat model of acute myocardial infarction. Control, control group; Isoflurane, isoflurane group; Low-dose, isoflurane + low-dose baicalin group (1 mg/mg); Medium-dose, isoflurane + medium-dose baicalin group (10 mg/mg); High-dose, isoflurane + high-dose baicalin group (100 mg/mg). #P < 0.01 compared with isoflurane group.
Figure 6
Figure 6
Baicalin ameliorates cellular apoptosis in a rat model of acute myocardial infarction. Control, control group; Isoflurane, isoflurane group; Low-dose, isoflurane + low-dose baicalin group (1 mg/mg); Medium-dose, isoflurane + medium-dose baicalin group (10 mg/mg); High-dose, isoflurane + high-dose baicalin group (100 mg/mg). #P < 0.01 compared with isoflurane group.
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