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. 2016 Jan 25;52(7):1405-8.
doi: 10.1039/c5cc05392g.

A fast click-slow release strategy towards the HPLC-free synthesis of RNA

E Agustin  1 , P N Asare Okai  1 , I Khan  1 , M R Miller  1 , R Wang  1 , J Sheng  1 , M Royzen  1
Affiliations

A fast click-slow release strategy towards the HPLC-free synthesis of RNA

E Agustin et al. Chem Commun (Camb). .

Abstract

A general strategy for purification of oligonucleotides synthesized by solid phase synthesis is described. It is based on a recently developed concept involving a bio-orthogonal inverse electron demand Diels-Alder reaction between trans-cyclooctene and tetrazine, termed 'click-to-release'. The strategy has been applied towards the synthesis and purification of a model hairpin RNA strand, as well as a 34 nt long aptamer.

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