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. 2015 Oct;10(4):2422-2426.
doi: 10.3892/ol.2015.3527. Epub 2015 Jul 23.

Effects of siRNA Livin on EJ human bladder cancer cells treated with mitomycin-C

Ya-Hui Song  1 , Ran Liao  1 , Peng-Cheng Li  1 , B O Ge  1 , Lei-Ming Jiang  1 , L I Gao  1 , Tian-Yu Zhang  1
Affiliations

Effects of siRNA Livin on EJ human bladder cancer cells treated with mitomycin-C

Ya-Hui Song et al. Oncol Lett. 2015 Oct.

Abstract

The aim of this study was to observe the inhibitory and therapeutic effects of small interfering RNA (siRNA) targeting Livin in EJ human bladder cancer cells. Specific siRNA targeting Livin was synthesized and transfected into EJ human bladder cancer cells treated or not treated with mitomycin-C (MMC). Livin mRNA and protein, as well as proliferation and apoptosis of EJ cells was examined with reverse transcription-polymerase chain reaction, western blotting, Cell Counting Kit-8 assay and flow cytometry, respectively. The results indicated that the expression of Livin mRNA and protein in EJ cells was significantly decreased by siRNA Livin. The proliferation of EJ cells was significantly inhibited by treatment with MMC and transfection of siRNA Livin. The inhibition of cell proliferation by treatment with MMC was further enhanced by transfection of siRNA Livin. The apoptotic rate of cells transfected with siRNA Livin and treated with MMC was significantly higher than those cells receiving a single transfection of siRNA Livin and single treatment of MMC. In conclusion, the present study demonstrates that transfection of siRNA Livin induces growth suppression and apoptosis in EJ human bladder cancer cells, and increases the chemotherapeutic sensitivity of cells to MMC.

Keywords: RNA interference; bladder cancer; chemotherapeutic sensitivity; mitomycin-C; small interfering RNA.

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Figures

Figure 1.
Figure 1.
EJ bladder carcinoma cells in blank control group (A) and transfected with negative siRNA Livin (B) under inverted fluorescence microscopy (magnification, ×400).
Figure 2.
Figure 2.
Expression of Livin mRNA (A) and protein (B) in different groups after 24 and 48 h transfection with siRNA Livin. **P<0.01 represents the relative Livin mRNA expression in blank control, negative control or lipofectamine groups compared with siRNA-treated cells.
Figure 3.
Figure 3.
Apoptosis of EJ cells in (A) blank control group, (B) intervention group (C) chemotherapy group and (D) combination group. (E) The difference among the four groups was also statistically analyzed. **P<0.01 represents the apoptotic cell percentage in the blank control, intervention and chemotherapy groups compared with the combination group.
See this image and copyright information in PMC

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