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. 2013 Nov 9:5:13-22.
doi: 10.1016/j.rinim.2013.10.003. eCollection 2015.

Predictive value of antinuclear antibodies in autoimmune diseases classified by clinical criteria: Analytical study in a specialized health institute, one year follow-up

María Elena Soto  1 , Nidia Hernández-Becerril  1 , Ada Claudia Perez-Chiney  1 , Alfredo Hernández-Rizo  1 , José Eduardo Telich-Tarriba  2 , Luis Eduardo Juárez-Orozco  3 , Gabriela Melendez  4 , Rafael Bojalil  5
Affiliations

Predictive value of antinuclear antibodies in autoimmune diseases classified by clinical criteria: Analytical study in a specialized health institute, one year follow-up

María Elena Soto et al. Results Immunol. .

Abstract

Introduction: Determination of antinuclear antibodies (ANA) by indirect immunofluorescence (IIF) is usually the initial test for the diagnosis of systemic rheumatic diseases (SRD). Assigning predictive values to positive and negative results of the test is vital because lack of knowledge about ANAs and their usefulness in classification criteria of SRD leads to inappropriate use.

Methods: Retrospective study, ANA tests requested by different specialties, correlation to patients' final diagnosis.

Results: The prevalence of autoimmune disease was relatively low in our population yielding a low PPV and a high NPV for the ANA test. 40% of the patients had no clinical criteria applied prior to test. Coexistence of two or more autoimmune disorders affects prevalence and predictive values.

Conclusion: Application of the test after careful evaluation for clinical criteria remarkably improves the positive likelihood ratio for the diagnosis.

Keywords: Antinuclear antibodies; Clinical criteria; Generalized rheumatic disease; Predictive value..

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Figures

Fig. 1
Fig. 1
Microphotographs of indirect immunofluorescence of specific antibodies in Hep2 cells. (a) Cytoplasmic P-ribosomal pattern. (b) NuMA-1 pattern. (c) Centromeric or discrete speckled pattern. (d) SSA discrete speckled pattern. (e) Homogeneous pattern. (f) Nucleolar with mitochondrial pattern.
See this image and copyright information in PMC

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