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. 2015 Sep 15;8(9):14962-8.
eCollection 2015.

Role of WNT1-inducible-signaling pathway protein 1 in etoposide resistance in lung adenocarcinoma A549 cells

Yunhua Xu  1 , Shun Lu  1
Affiliations

Role of WNT1-inducible-signaling pathway protein 1 in etoposide resistance in lung adenocarcinoma A549 cells

Yunhua Xu et al. Int J Clin Exp Med. .

Abstract

Object: The aim of this study was to explore the role of WNT1-inducible-signaling Pathway Protein 1 (WISP-1) in etoposide resistance in lung adenocarcinoma A549 cells.

Methods: WISP-1 overexpression A549 lung adenocarcinoma cell was established. After exposure to ultraviolet (UV) and etoposide, cell viability and apoptosis were evaluated. Moreover, western-blot was employed to examine the expression of apoptotic pathway proteins. In addition, a nude mice tumor model was established to examine the effect of WISP-1 overexpression in vivo and TUNEL staining was used to assess cell apoptosis of tumor tissue.

Results: WISP-1 overexpression significantly increased cell viability and decreased cell apoptosis after treatment with UV and etoposide. Decreased expression of Bad and Bax and increased expression of Bcl-2 was found after etoposide treatment in WISP-1 overexpressed cells. A significantly increasing of tumor volume in WISP-1 overexpressed group was found and TUNEL staining revealed that decreased cell apoptosis in WISP-1 overexpressed group.

Conclusion: Our results demonstrated that WISP-1 may have a facilitating role in etoposide resistance through increasing cell viability and decreasing cell apoptosis.

Keywords: A549; WNT1-inducible-signaling pathway protein 1; apoptosis; etoposide; lung adenocarcinoma.

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Figures

Figure 1
Figure 1
WISP-1 overexpression obviously improved cell viability. Cell morphology of WISP-1overexpressed lung adenocarcinoma A549 cells after treatment with ultraviolet (UV) radiation or etoposide. A549 cells were treated with UV light at 50 J/m2 for 24 hrs or with etoposide at 50 μM for 24 hrs.
Figure 2
Figure 2
WISP-1 overexpression significantly decreased lung adenocarcinoma A549 cell apoptosis induced by ultraviolet radiation or etoposide.
Figure 3
Figure 3
The expression of apoptosis pathway protein in WISP-1 overexpressed lung adenocarcinoma A549 cells. Etoposide can decrease the expression of Bad, Bax expression, and increase the expression of Bcl-2.
Figure 4
Figure 4
WISP-1 overexpression on tumor growth inhibition by etoposide in a nude mice model. A. Etoposide treatment could significantly suppress the A549 cell growth in a nude mice while WISP overexpression partially restored the cancer cell growth. B. Time course of tumor volume in different treatment group. A549 cells (2 × 106 cells) were transplanted subcutaneously and tumor volume was measured.
Figure 5
Figure 5
Cell apoptosis of tumor tissue derived from a lung adenocarcinoma A549 subcutaneous transplantation nude mice model. TUNEL staining was used to determine the cell apoptosis.
See this image and copyright information in PMC

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