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. 2015:2015:827879.
doi: 10.1155/2015/827879. Epub 2015 Nov 8.

The Influence of α-Lipoic Acid and Garlic Administration on Biomarkers of Oxidative Stress and Inflammation in Rabbits Exposed to Oxidized Nutrition Oils

Jolanta Zalejska-Fiolka  1 , Tomasz Wielkoszyński  1 , Wojciech Rokicki Jr  2 , Natalia Dąbrowska  3 , Joanna Katarzyna Strzelczyk  3 , Aleksandra Kasperczyk  1 , Aleksander Owczarek  4 , Urszula Błaszczyk  1 , Sławomir Kasperczyk  1 , Barbara Stawiarska-Pięta  5 , Ewa Birkner  1 , Andrzej Gamian  6
Affiliations

The Influence of α-Lipoic Acid and Garlic Administration on Biomarkers of Oxidative Stress and Inflammation in Rabbits Exposed to Oxidized Nutrition Oils

Jolanta Zalejska-Fiolka et al. Biomed Res Int. 2015.

Abstract

We hypothesized that addition of substances with antioxidant activity could decrease the concentrations of biomarkers of oxidative stress and inflammatory process, thus inhibiting nonalcoholic steatohepatitis development. We investigated the influence of α-lipoic acid (ALA) and garlic administration on the development of adverse changes in rabbit liver and serum under oxidative stress conditions induced with HFD from oxidized oils. We determined 8-hydroxy-2'-deoxyguanosine (8 OHdG) and malondialdehyde (MDA) in liver homogenates, total oxidant status (TOS), lipid peroxides (LOO) and tumor necrosis factor alpha (TNFα) in blood serum, and TNFα and IL-1α genes expression in liver. The results indicate that the intake of dietary ALA and garlic was significantly associated with decreases of 8 OHdG and MDA levels in rabbits' liver tissue as well as TOS and LOO levels in rabbits' serum. Similarly, TNFα and IL-1α gene expressions were suppressed due to ALA and garlic supplementation. The histopathological analysis confirmed that HFD results in liver disorder leading to steatosis. This adverse effect of HFD was ameliorated by the supplementation of ALA and garlic. The obtained results indicate a beneficial effect of ALA and garlic administration by reducing the oxidative stress intensity and the levels of some proinflammatory cytokines in rabbits fed HFD.

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Figures

Figure 1
Figure 1
Malondialdehyde concentrations in the liver of rabbits in all study groups.
Figure 2
Figure 2
Level of 8-hydroxy-2′-deoxyguanosine in DNA of rabbits in all study groups.
Figure 3
Figure 3
TNFα gene expression in all study groups.
Figure 4
Figure 4
IL-1α gene expression in all study groups.
Figure 5
Figure 5
Liver tissue microphotography of controls: there are no abnormal changes of hepatocytes. H-E staining, 200x.
Figure 6
Figure 6
Liver tissue microphotography of oxidized rapeseed oil group. Fatty hepatocytes and focal necrotic lesions are present. H-E staining, 200x.
Figure 7
Figure 7
Liver tissue microphotography of oxidized olive oil group. Mononuclear cells infiltration around the triad area. H-E staining, 200x.
See this image and copyright information in PMC

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