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. 1989 Apr;49(2-3):139-43.
doi: 10.1016/0378-1097(89)90028-1.

Low level expression in Escherichia coli of a fungal gene under the control of strong promoters

C Patiño  1 , F Sánchez, M A Peñalva
Affiliations

Low level expression in Escherichia coli of a fungal gene under the control of strong promoters

C Patiño et al. FEMS Microbiol Lett. 1989 Apr.

Abstract

We have chosen the Isopenicillin N synthetase (IPNS) gene from Cephalosporium acremonium to study its expression in Escherichia coli due to its peculiar DNA sequence [1]. Significant levels of the protein could not be detected when the IPNS gene was placed under the control of strong promoters. Only when the construction was cloned in a runaway plasmid, significant levels of the protein were found in lysates of E. coli. Besides, the presence of the IPNS gene inhibits expression of a distal gene (galK) in a polycistronic RNA, suggesting that the gene has a low transcriptional efficiency in E. coli.

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