Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Dec 3;8(3):729-32.
doi: 10.1093/gbe/evv246.

Draft Genome Sequences of Two Closely Related Aflatoxigenic Aspergillus Species Obtained from the Ivory Coast

Geromy G Moore  1 , Brian M Mack  2 , Shannon B Beltz  2
Affiliations

Draft Genome Sequences of Two Closely Related Aflatoxigenic Aspergillus Species Obtained from the Ivory Coast

Geromy G Moore et al. Genome Biol Evol. .

Abstract

Aspergillus ochraceoroseus and Aspergillus rambellii were isolated from soil detritus in Taï National Park, Ivory Coast, Africa. The Type strain for each species happens to be the only representative ever sampled. Both species secrete copious amounts of aflatoxin B1 and sterigmatocystin, because each of their genomes contains clustered genes for biosynthesis of these mycotoxins. We sequenced their genomes using a personal genome machine and found them to be smaller in size (A. ochraceoroseus = 23.9 Mb and A. rambellii = 26.1 Mb), as well as in numbers of predicted genes (7,837 and 7,807, respectively), compared to other sequenced Aspergilli. Our findings also showed that the A. ochraceoroseus Type strain contains a single MAT1-1 gene, while the Type strain of A. rambellii contains a single MAT1-2 gene, indicating that these species are heterothallic (self-infertile). These draft genomes will be useful for understanding the genes and pathways necessary for the cosynthesis of these two toxic secondary metabolites as well as the evolution of these pathways in aflatoxigenic fungi.

Keywords: Aspergillus ochraceoroseus, Aspergillus rambellii; genome sequence; mating-type locus; phylogenomics.

PubMed Disclaimer

Figures

F<sc>ig</sc>. 1.—
Fig. 1.—
Phylogenomic comparison of sequenced Aspergillus species reveals patterns of ancestry. This tree was inferred using multiple AF and/or ST producing Aspergillus species (A. flavus L, A. nidulans, A. nomius, A. ochraceoroseus, and A. rambellii) and one AF and ST nonproducing species (A. fumigatus), with Penicillium chrysogenum as the outgroup taxa.
See this image and copyright information in PMC

References

    1. Bartoli A, Maggi O. 1978. Four new species of Aspergillus from Ivory Coast soil. Trans Br Mycol Soc. 71:383–394.
    1. Cary JW, Ehrlich KC, Beltz SB, Harris-Coward P, Klich MA. 2009. Characterization of the Aspergillus ochraceoroseus aflatoxin/sterigmatocystin biosynthetic gene cluster. Mycologia 101:352–362. - PubMed
    1. Cary JW, et al. 2012. Functional and phylogenetic analysis of the Aspergillus ochraceoroseus aflQ (ordA) gene ortholog. Mycologia. 104:857–864. - PubMed
    1. Ehrlich KC, Yu J, Cotty PJ. 2005. Aflatoxin biosynthesis gene clusters and flanking regions. J Appl Microbiol. 99:518–527. - PubMed
    1. Frisvad JC, Skouboe P, Samson RA. 2005. Taxonomic comparison of three different groups of aflatoxin producers and a new efficient producer of aflatoxin B1, sterigmatocystin and 3-O-methylsterigmatocystin, Aspergillus rambellii sp. nov. Sys Appl Microbiol. 28:442–453. - PubMed

Publication types