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. 2016 Dec;53(10):6668-6679.
doi: 10.1007/s12035-015-9553-z. Epub 2015 Dec 8.

SVCT2 Overexpression in Neuroblastoma Cells Induces Cellular Branching that is Associated with ERK Signaling

Katterine Salazar  1 , Milka Martínez  1 , Viviana Ulloa  1 , Romina Bertinat  1 , Fernando Martínez  1 , Nery Jara  1 , Francisca Espinoza  1 , Ernesto R Bongarzone  2 , Francisco Nualart  3
Affiliations

SVCT2 Overexpression in Neuroblastoma Cells Induces Cellular Branching that is Associated with ERK Signaling

Katterine Salazar et al. Mol Neurobiol. 2016 Dec.

Abstract

Expression of the sodium and ascorbic acid (AA) cotransporter SVCT2 is induced during the period of cellular arborization and synaptic maturation of early postnatal (P1-P5) rat cerebral neurons. The physiological importance of the transporter for neurons is evidenced by the lethality and delayed neuronal differentiation detected in mice with ablation of SVCT2. The mechanism(s) involved in these defects and the role of SVCT2 in neuronal branching have not been determined yet. To address this, we used lentiviral expression vectors to increase the levels of SVCT2 in N2a cells and analyzed the effects on neurite formation. Expression of a fusion protein containing the human SVCT2wt and EYFP induced an increase in the number of MAP2+ neurites and filopodia in N2a cells. Overexpression of SVCT2 and treatment with AA promoted ERK1/2 phosphorylation. Our data suggest that enhanced expression of the high affinity AA transporter SVCT2, which tightly regulates intracellular AA concentrations, induces neuronal branching that then activates key signaling pathways that are involved in the differentiation and maturation of cortical neurons during postnatal development.

Keywords: Branching; Filopodia; N2a cells; SVCT1; SVCT2; Vitamin C.

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References

    1. Cell Death Differ. 2007 Oct;14(10):1792-801 - PubMed
    1. Nat Rev Neurosci. 2004 Mar;5(3):173-83 - PubMed
    1. Am J Physiol Endocrinol Metab. 2010 Aug;299(2):E325-34 - PubMed
    1. Oncogene. 1999 Feb 11;18(6):1285-96 - PubMed
    1. Nat Med. 2002 May;8(5):514-7 - PubMed

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