Association of Genetic Polymorphisms of IFNGR1 with the Risk of Pulmonary Tuberculosis in Zahedan, Southeast Iran

Abstract

Aim. The present study was undertaken to find out the possible association between interferon-gamma (IFN-γ) receptor 1 (IFNGR1) gene polymorphisms and risk of pulmonary tuberculosis (PTB) in a sample of Iranian population. Methods. Polymorphisms of IFNGR1 rs1327474 (-611 A/G), rs11914 (+189 T/G), rs7749390 (+95 C/T), and rs137854905 (27-bp ins/del) were determined in 173 PTB patients and 164 healthy subjects. Results. Our findings showed that rs11914 TG genotypes decreased the risk of PTB in comparison with TT (OR = 0.36, 95% CI = 0.21-0.62, and p = 0.0002). The rs11914 G allele decreased the risk of PTB compared with T allele (OR = 0.41, 95% CI = 0.25-0.68, and p = 0.0006). IFNGR1 rs7749390 CT genotype decreased the risk of PTB in comparison with CC genotype (OR = 0.55, 95% CI = 0.32-0.95, and p = 0.038). No significant association was found between IFNGR1 rs1327474 A/G polymorphism and risk/protective of PTB. The rs137854905 (27-bp I/D) variant was not polymorphic in our population. Conclusion. Our findings showed that IFNGR1 rs11914 and rs7749390 variants decreased the risk of PTB susceptibility in our population.

Figure 1

Electrophoresis pattern of polymerase chain reaction restriction fragments length polymorphism (PCR-RFLP) for detection of IFNGR1 rs1327474 polymorphism. G allele digested by Hpy188I restriction enzyme and produces 204 and 29 bp while A allele undigested (233 bp). M: DNA marker; lanes 1, 2, and 5: AA; lane 3: GG; lane 4: AG.

Figure 2

Electrophoresis pattern of PCR-RFLP for detection of IFNGR1 rs11914 polymorphism. G allele digested by TaqI restriction enzyme and produces 286 and 210 bp while the T allele undigested (496 bp). M: DNA marker; lanes 1, 2, 4, and 5: TT; lane 3: TG.

Figure 3

Electrophoresis pattern of PCR-RFLP for detection of IFNGR1 rs7749390 polymorphism. The 366-bp PCR product digested by BstC8I. C allele produces 87, 121, and 158 bp, while the T allele produces 208 and 158 bp. M: DNA marker; lanes 1 and 2: CC; lanes 3 and 4: CT; Lane 5: TT.

Figure 4

Electrophoresis pattern of PCR for detection of IFNGR1 rs137854905 (27-bp I/D) polymorphism. I allele produces 223 bp while D allele produces 196 bp. M: DNA marker; lanes 1, 2, 3, 4, and 5: DD.