doi: 10.1016/0378-1119(89)90174-1.
Participation of the hup gene product in site-specific DNA inversion in Escherichia coli
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- PMID: 2666260
- DOI: 10.1016/0378-1119(89)90174-1
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Participation of the hup gene product in site-specific DNA inversion in Escherichia coli
Gene.
1989.
Abstract
The closely related Escherichia coli genes hupA and hupB each encode a bacterial histone-like protein HU. We report here that DNA inversion mediated by hin, gin, pin and rci but not by cin is blocked in a hupA hupB double mutant, although inversions in these systems occur in the hupA or hupB single mutant as efficiently as in the wild-type strain. These findings show that HU protein participates in site-specific DNA inversion in E. coli and that only one subunit, either HU-1 or HU-2, is sufficient for this inversion.
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