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. 1977 May;74(5):1899-902.
doi: 10.1073/pnas.74.5.1899.

Translation in vitro of rat liver messenger RNA coding for ligandin (glutathione S-transferase B)

V Daniel, G J Smith, G Litwack

Translation in vitro of rat liver messenger RNA coding for ligandin (glutathione S-transferase B)

V Daniel et al. Proc Natl Acad Sci U S A. 1977 May.

Abstract

Poly(A)-containing rat liver mRNA isolated from animals injected with phenobarbital and uninjected controls was translated efficiently in a wheat-germ system. The synthesis of ligandin (glutathione S-transferase B; glutathione transferase; RX-gluathione R-transferase, EC 2.5.1.18) was detected by immunoprecipitation with a highly purified monospecific ligandin antibody and analysis by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. The extent of incorporation of [35S]methionine into ligandin in the translation system was similar for poly(A)-containing messages from un-infected animals and those treated with phenobarbital.

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