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. 1989 Jul 11;17(13):5361-75.
doi: 10.1093/nar/17.13.5361.

Scission of RNA by the chemical nuclease of 1,10-phenanthroline-copper ion: preference for single-stranded loops

G J Murakawa  1 , C H Chen, M D Kuwabara, D P Nierlich, D S Sigman
Affiliations
Free PMC article

Scission of RNA by the chemical nuclease of 1,10-phenanthroline-copper ion: preference for single-stranded loops

G J Murakawa et al. Nucleic Acids Res. .
Free PMC article

Abstract

The scission of RNA by the chemical nuclease activity of 1,10-phenanthroline-copper (OP-Cu) has been studied using a lac mRNA fragment and tRNAphe as substrates. Since the chemical mechanism of scission involves oxidative attack on the ribose, scission is observed at all nucleotides including dihydrouridine and Y-bases. Specificity for single-stranded loop regions is apparent from the similarity of the reactivity of OP-Cu to the single-strand specific reagents dimethyl sulfate and diethyl pyrocarbonate using the fragment of lac mRNA as a substrate. Similar preference is observed in the reaction with tRNA although scission in the helical acceptor stem is also observed.

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