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. 2015 Dec 21:15:443.
doi: 10.1186/s12906-015-0961-4.

Copaifera langsdorffii oleoresin and its isolated compounds: antibacterial effect and antiproliferative activity in cancer cell lines

Fariza Abrão  1 , Luciana Delfino de Araújo Costa  1 , Jacqueline Morais Alves  2 , Juliana Marques Senedese  2 , Pâmela Tinti de Castro  2 , Sérgio Ricardo Ambrósio  3 , Rodrigo Cássio Sola Veneziani  3 , Jairo Kenupp Bastos  4 , Denise Crispim Tavares  2 , Carlos Henrique G Martins  5
Affiliations

Copaifera langsdorffii oleoresin and its isolated compounds: antibacterial effect and antiproliferative activity in cancer cell lines

Fariza Abrão et al. BMC Complement Altern Med. .

Abstract

Background: Natural products display numerous therapeutic properties (e.g., antibacterial activity), providing the population with countless benefits. Therefore, the search for novel biologically active, naturally occurring compounds is extremely important. The present paper describes the antibacterial action of the Copaifera langsdorffii oleoresin and ten compounds isolated from this oleoresin against multiresistant bacteria; it also reports the antiproliferative activity of the Copaifera langsdorffii oleoresin and (-)-copalic acid.

Methods: MICs and MBCs were used to determine the antibacterial activity. Time-kill curve assays provided the time that was necessary for the bacteria to die. The Minimum Inhbitory Concentration of Biofilm (CIMB50) of the compounds that displayed the best results was calculated. Cytotoxicity was measured by using the XTT assay.

Results: The diterpene (-)-copalic acid was the most active antibacterial and afforded promising Minimum Inhibitory Concentration (MIC) values for most of the tested strains. Determination of the bactericidal kinetics against some bacteria revealed that the bactericidal effect emerged within six hours of incubation for Streptococcus pneumoniae. Concerning the antibiofilm action of this diterpene, its MICB50 was twofold larger than its CBM against S. capitis and S. pneumoniae. The XTT assay helped to evaluate the cytotoxic effect; results are expressed as IC50. The most pronounced antiproliferative effect arose in tumor cell lines treated with (-)-copalic acid; the lowest IC50 value was found for the human glioblastoma cell line.

Conclusions: The diterpene (-)-copalic acid is a potential lead for the development of new selective antimicrobial agents to treat infections caused by Gram-positive multiresistant microorganisms, in both the sessile and planktonic mode. This diterpene is also a good candidate to develop anticancer drugs.

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Figures

Fig. 1
Fig. 1
Chemical structures of the evaluated compounds
Fig. 2
Fig. 2
Chemical structures of the methylated compounds
Fig. 3
Fig. 3
Bactericidal kinetics of copalic acid against multiresistant bacteria
Fig 4
Fig 4
Antibiofilm activity of Copalic acid as demonstrated by optical density (A570) and number of microorganisms (Log10 CFU mL−1)
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References

    1. Livermore DM. Fourteen years in resistance. Int J Antimicrob. 2012;39(4):283–94. doi: 10.1016/j.ijantimicag.2011.12.012. - DOI - PubMed
    1. Gibbons S. Phytochemicals for bacterial resistance – Strengths, weaknesses and opportunities. Planta Med. 2008;74(6):594–602. doi: 10.1055/s-2008-1074518. - DOI - PubMed
    1. Kumar VP, Chauhan NS, Padh H, Rajani M. Search for antibacterial and antifungal agents from selected Indian medicinal plants. J Ethnopharmacol. 2006;107(2):182–8. doi: 10.1016/j.jep.2006.03.013. - DOI - PubMed
    1. Solórzano-Santos F, Miranda-Novales MG. Essential oils from aromatic herbs as antimicrobial agents. Curr Opin Biotechnol. 2012;23(2):136–41. doi: 10.1016/j.copbio.2011.08.005. - DOI - PubMed
    1. Simões M, Simões LC, Vieira MJ. A review of current and emergent biofilm control strategies. Food Sci Technol. 2010;43(4):573–83.

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