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. 2015 Dec 23:14:55.
doi: 10.1186/s12941-015-0114-0.

Clinical features and characteristics of Clostridium difficile PCR-ribotype 176 infection: results from a 1-year university hospital internal ward study

Jiri Drabek  1 , Otakar Nyc  2 , Marcela Krutova  3   4 , Jan Stovicek  5 , Jana Matejkova  6 , Radan Keil  7
Affiliations

Clinical features and characteristics of Clostridium difficile PCR-ribotype 176 infection: results from a 1-year university hospital internal ward study

Jiri Drabek et al. Ann Clin Microbiol Antimicrob. .

Abstract

Background: Clostridium difficile infection (CDI) is a major cause of antibiotic-associated diarrhoea. Given an increasing CDI incidence and global spread of epidemic ribotypes, a 1-year study was performed to analyse the molecular characteristics of C. difficile isolates and associated clinical outcomes from patients diagnosed with CDI in the Internal Medicine department at University Hospital Motol, Prague from February 2013 to February 2014.

Results: A total of 85 unformed stool samples were analysed and CDI was laboratory confirmed in 30 patients (6.8 CDI cases per 10,000 patient bed days and 50.6 CDI cases per 10,000 admissions). The CDI recurrence rate within 3 months of treatment discontinuation was 13.3% (4/30). Mortality within 3 months after first CDI episode was 26.7% (8/30), with CDI the cause of death in two cases. 51.9% of C. difficile isolates belonged to PCR-ribotype 176. MLVA of ribotype 176 isolates revealed two clonal complexes formed by 10/14 isolates. ATLAS scores and Horn's index were higher in patients with ribotype 176 infections than with non-ribotype 176 infections.

Conclusion: This study highlights the clinical relevance of C. difficile PCR-ribotype 176 and its capacity to spread within a healthcare facility.

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Figures

Fig. 1
Fig. 1
A Categorical MLVA of C. difficile ribotype 176 isolates (Bionumerics v5.0, Applied Maths)
Fig. 2
Fig. 2
A Minimum Spanning Tree of C. difficile ribotype 176 isolates (Bionumerics v5.0, Applied Maths). The numbers in the circles represent C. difficile PCR-ribotype 176 isolate number. The numbers in the lines represent the sum of tandem repeat differences between isolates
See this image and copyright information in PMC

References

    1. Kuijper EJ, Coignard B, Tüll P, ESCMID Study Group for Clostridium difficile EU Member States; European Centre for Disease Prevention and Control. Emergence of Clostridium difficile-associated disease in North America and Europe. Clin Microbiol Infect. 2006;12(Suppl 6):2–18. doi: 10.1111/j.1469-0691.2006.01580.x. - DOI - PubMed
    1. Bauer MP, Notermans DW, van Benthem BH, Brazier JS, Wilcox MH, Rupnik M, Monnet DL, van Dissel JT, Kuijper EJ, ECDIS Study Group Clostridium difficile infection in Europe: a hospital-based survey. Lancet. 2011;377(9759):63–73. doi: 10.1016/S0140-6736(10)61266-4. - DOI - PubMed
    1. Davies KA, Longshaw CM, Davis GL, Bouza E, Barbut F, Barna Z, Delmée M, Fitzpatrick F, Ivanova K, Kuijper E, Macovei IS, Mentula S, Mastrantonio P, von Müller L, Oleastro M, Petinaki E, Pituch H, Norén T, Nováková E, Nyč O, Rupnik M, Schmid D, Wilcox MH. Underdiagnosis of Clostridium difficile across Europe: the European, multicentre, prospective, biannual, point-prevalence study of Clostridium difficile infection in hospitalised patients with diarrhoea (EUCLID) Lancet Infect Dis. 2014;14(12):1208–1219. doi: 10.1016/S1473-3099(14)70991-0. - DOI - PubMed
    1. Krutova M, Nyc O, Kuijper EJ, Geigerova L, Matejkova J, Bergerova T, Arvand M. A case of imported Clostridium difficile PCR-ribotype 027 infection within the Czech Republic which has a high prevalence of C. difficile ribotype 176. Anaerobe. 2014;30:153–155. doi: 10.1016/j.anaerobe.2014.09.020. - DOI - PubMed
    1. Valiente E, Cairns MD, Wren BW. The Clostridium difficile PCR ribotype 027 lineage: a pathogen on the move. Clin Microbiol Infect. 2014;20(5):396–404. doi: 10.1111/1469-0691.12619. - DOI - PubMed

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