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. 2016 Mar;54(3):768-70.
doi: 10.1128/JCM.02035-15. Epub 2015 Dec 23.

Two Listeria monocytogenes Pseudo-outbreaks Caused by Contaminated Laboratory Culture Media

Almea Matanock  1 , Lee S Katz  2 , Kelly A Jackson  2 , Zuzana Kucerova  2 , Amanda R Conrad  3 , William A Glover  4 , Von Nguyen  2 , Marika C Mohr  5 , Nicola Marsden-Haug  4 , Deborah Thompson  6 , John R Dunn  7 , Steven Stroika  2 , Beth Melius  4 , Cheryl Tarr  2 , Stephen E Dietrich  8 , Annie S Kao  9 , Laura Kornstein  10 , Zhen Li  4 , Azarnoush Maroufi  9 , Ellyn P Marder  7 , Rebecca Meyer  11 , Ailyn C Perez-Osorio  4 , Vasudha Reddy  10 , Roshan Reporter  12 , Heather Carleton  2 , Samantha Tweeten  9 , HaeNa Waechter  10 , Lisa M Yee  9 , Matthew E Wise  2 , Kim Davis  13 , Brendan R Jackson  2
Affiliations

Two Listeria monocytogenes Pseudo-outbreaks Caused by Contaminated Laboratory Culture Media

Almea Matanock et al. J Clin Microbiol. 2016 Mar.

Abstract

Listeriosis is a serious foodborne infection that disproportionately affects elderly adults, pregnant women, newborns, and immunocompromised individuals. Diagnosis is made by culturing Listeria monocytogenes from sterile body fluids or from products of conception. This report describes the investigations of two listeriosis pseudo-outbreaks caused by contaminated laboratory media made from sheep blood.

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Figures

FIG 1
FIG 1
Relationships by whole-genome multilocus sequencing type (wgMLST) of Listeria monocytogenes isolates with three pulsed-field gel electrophoresis (PFGE) patterns from 11 U.S. states associated with the 2014 pseudo-outbreak. Median (minimum to maximum) allele differences at node by wgMLST analysis performed using BioNumerics 7.5 and alleles were defined using an allele database generated to reflect the diversity of L. monocytogenes (Listeria database v.3). *, Isolates from uninoculated laboratory media plates; +, Isolates excluded from the pseudo-outbreak because of a clinical history compatible with listeriosis.
See this image and copyright information in PMC

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