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. 2016 Apr;38(4):667-72.
doi: 10.1007/s10529-015-2019-6. Epub 2015 Dec 23.

Morphology engineering of basidiomycetes for improved laccase biosynthesis

Anna Antecka  1 , Michał Blatkiewicz  2 , Marcin Bizukojć  2 , Stanisław Ledakowicz  2
Affiliations

Morphology engineering of basidiomycetes for improved laccase biosynthesis

Anna Antecka et al. Biotechnol Lett. 2016 Apr.

Abstract

Objective: This work is the first application of a morphological engineering technique called microparticle-enhanced cultivation (MPEC) aimed at the facilitation of laccase production in the submerged cultures by two basidiomycetes species Cerrena unicolor and Pleurotus sapidus.

Results: The positive effect of the applied 10 μm Al2O3 microparticles at concentrations from 5 to 30 g Al2O3 l(-1) was shown. Laccase activity increased 3.5-fold for C. unicolor and 2-fold for P. sapidus at 15 g Al2O3 l(-1) on 9 and 14 day of the cultivation, respectively, compared to the control culture without microparticles. The increase of laccase activity in the cultivation broths was caused by the action of Al2O3 microparticles on the agglomeration of hyphae. It led to the decrease of the size of the pellets, (on average by 2 mm for C. unicolor), the change of their shape (star-shaped pellets for C. unicolor) and the change of their structure (more compact pellets for P. sapidus).

Conclusions: Application of MPEC for the submerged cultures of two laccase-producing basidiomycetes proved successful in increasing of enzyme production.

Keywords: Basidiomycetes; Fungal morphology; Laccase; Microparticle-enhanced cultivation.

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Figures

Fig. 1
Fig. 1
Influence of Al2O3 microparticles concentration on laccase activity for a C. unicolor and b P. sapidus; the presented points represent the averaged values from three experiments and error bars are standard deviation
Fig. 2
Fig. 2
Time changes of laccase activity at various Al2O3 concentration added to the culture of a C. unicolor and b P. sapidus; the presented points represent the averaged values from three experiments and error bars are standard deviation
Fig. 3
Fig. 3
Influence of Al2O3 microparticles added to the culture medium on C. unicolor morphology: a control run without microparticles, b 15 g Al2O3 l−1 and c 30 g Al2O3 l−1; scale-bar size 1 cm
Fig. 4
Fig. 4
Influence of Al2O3 microparticles added to the culture medium on P. sapidus morphology: a control run without microparticles, b 15 g Al2O3 l−1 and c 30 g Al2O3 l−1; scale-bar size 1 cm
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