Both CheA and CheW are required for reconstitution of chemotactic signaling in Escherichia coli
- PMID: 2670907
- PMCID: PMC210339
- DOI: 10.1128/jb.171.9.5190-5193.1989
Both CheA and CheW are required for reconstitution of chemotactic signaling in Escherichia coli
Abstract
If cells of Escherichia coli deleted for genes that specify transducers and all known cytoplasmic chemotaxis proteins are reconstituted with CheA, CheW, and CheY, they spin their flagella alternately clockwise and counterclockwise. If the aspartate receptor also is present, clockwise rotation is suppressed upon addition of aspartate. If either CheA or CheW is absent, the fraction of time that the flagella spin clockwise is reduced and responses to aspartate do not occur.
Similar articles
-
Reconstitution of signaling in bacterial chemotaxis.J Bacteriol. 1987 May;169(5):1878-85. doi: 10.1128/jb.169.5.1878-1885.1987. J Bacteriol. 1987. PMID: 3553150 Free PMC article.
-
Mutants defective in bacterial chemotaxis show modified protein phosphorylation.Cell. 1988 Apr 8;53(1):89-96. doi: 10.1016/0092-8674(88)90490-4. Cell. 1988. PMID: 2832069
-
Conserved aspartate residues and phosphorylation in signal transduction by the chemotaxis protein CheY.Proc Natl Acad Sci U S A. 1990 Jan;87(1):41-5. doi: 10.1073/pnas.87.1.41. Proc Natl Acad Sci U S A. 1990. PMID: 2404281 Free PMC article.
-
Bacterial chemotaxis coupling protein: Structure, function and diversity.Microbiol Res. 2019 Feb;219:40-48. doi: 10.1016/j.micres.2018.11.001. Epub 2018 Nov 6. Microbiol Res. 2019. PMID: 30642465 Review.
-
Throwing the switch in bacterial chemotaxis.Trends Microbiol. 1999 Jan;7(1):16-22. doi: 10.1016/s0966-842x(98)01409-7. Trends Microbiol. 1999. PMID: 10068993 Review.
Cited by
-
Role of CheW protein in coupling membrane receptors to the intracellular signaling system of bacterial chemotaxis.Proc Natl Acad Sci U S A. 1989 Nov;86(22):8703-7. doi: 10.1073/pnas.86.22.8703. Proc Natl Acad Sci U S A. 1989. PMID: 2682657 Free PMC article.
-
Computer simulation of the phosphorylation cascade controlling bacterial chemotaxis.Mol Biol Cell. 1993 May;4(5):469-82. doi: 10.1091/mbc.4.5.469. Mol Biol Cell. 1993. PMID: 8334303 Free PMC article.
-
Change in direction of flagellar rotation in Escherichia coli mediated by acetate kinase.J Bacteriol. 1993 May;175(10):3236-9. doi: 10.1128/jb.175.10.3236-3239.1993. J Bacteriol. 1993. PMID: 8098330 Free PMC article.
-
Calcium ions are involved in Escherichia coli chemotaxis.Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):11804-8. doi: 10.1073/pnas.89.24.11804. Proc Natl Acad Sci U S A. 1992. PMID: 1465403 Free PMC article.
-
Bacterial chemotaxis signaling complexes: formation of a CheA/CheW complex enhances autophosphorylation and affinity for CheY.Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):6269-73. doi: 10.1073/pnas.88.14.6269. Proc Natl Acad Sci U S A. 1991. PMID: 2068106 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Molecular Biology Databases