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. 1989 Sep;171(9):5215-7.
doi: 10.1128/jb.171.9.5215-5217.1989.

pepM is an essential gene in Salmonella typhimurium

C G Miller  1 , A M Kukral, J L Miller, N R Movva
Affiliations

pepM is an essential gene in Salmonella typhimurium

C G Miller et al. J Bacteriol. 1989 Sep.

Abstract

The pepM gene of Salmonella typhimurium codes for a methionine-specific aminopeptidase that removes N-terminal methionine residues from proteins. This gene was inactivated in vitro by the insertion of a DNA fragment coding for kanamycin resistance. The inactivated gene could not replace the wild-type chromosomal pepM gene unless another functional copy was present in the cell. The lethal effect of the pepM insertion was not a result of polarity on any gene downstream, nor was it affected by the presence or absence of other peptidases.

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