Comparison of molecular and extract-based allergy diagnostics with multiplex and singleplex analysis

Affiliations

¹ Allergy Research Group, Department of Dermatology, Medical Center, University of Freiburg, Freiburg, Germany ; Thermo Fisher Scientific, Phadia GmbH, Freiburg, Germany ; AbbVie Deutschland GmbH & Co. KG, Mainzer Straße 81, 65189 Wiesbaden, Germany.
² Department of Dermatology, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel (VUB), Brussels, Belgium.
³ Thermo Fisher Scientific, Phadia GmbH, Freiburg, Germany.
⁴ Department of Dermatology and Allergy, Technische Universität München, Munich, Germany ; ZAUM-Center for Allergy and Environment, Helmholtz Center Munich, Technische Universität München, Munich, Germany.
⁵ Department of Dermatology and Allergy, Technische Universität München, Munich, Germany.
⁶ ZAUM-Center for Allergy and Environment, Helmholtz Center Munich, Technische Universität München, Munich, Germany.
⁷ Allergy Research Group, Department of Dermatology, Medical Center, University of Freiburg, Freiburg, Germany.
⁸ University Freiburg Medical Center, Department of Exercise Medicine and Sport, University of Freiburg, Freiburg, Germany ; Department of Pneumology, University of Freiburg, Freiburg, Germany ; Department of Pneumology, Freiburg University Medical Center, Albert-Ludwigs-University, Freiburg, Germany.

PMID: 26709369
PMCID: PMC4686215
DOI: 10.1007/s40629-015-0046-4

Comparison of molecular and extract-based allergy diagnostics with multiplex and singleplex analysis

Johannes Huss-Marp et al. Allergo J Int. 2015.

. 2015:24:46-53.

doi: 10.1007/s40629-015-0046-4. Epub 2015 Mar 14.

Authors

Affiliations

¹ Allergy Research Group, Department of Dermatology, Medical Center, University of Freiburg, Freiburg, Germany ; Thermo Fisher Scientific, Phadia GmbH, Freiburg, Germany ; AbbVie Deutschland GmbH & Co. KG, Mainzer Straße 81, 65189 Wiesbaden, Germany.
² Department of Dermatology, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel (VUB), Brussels, Belgium.
³ Thermo Fisher Scientific, Phadia GmbH, Freiburg, Germany.
⁴ Department of Dermatology and Allergy, Technische Universität München, Munich, Germany ; ZAUM-Center for Allergy and Environment, Helmholtz Center Munich, Technische Universität München, Munich, Germany.
⁵ Department of Dermatology and Allergy, Technische Universität München, Munich, Germany.
⁶ ZAUM-Center for Allergy and Environment, Helmholtz Center Munich, Technische Universität München, Munich, Germany.
⁷ Allergy Research Group, Department of Dermatology, Medical Center, University of Freiburg, Freiburg, Germany.
⁸ University Freiburg Medical Center, Department of Exercise Medicine and Sport, University of Freiburg, Freiburg, Germany ; Department of Pneumology, University of Freiburg, Freiburg, Germany ; Department of Pneumology, Freiburg University Medical Center, Albert-Ludwigs-University, Freiburg, Germany.

PMID: 26709369
PMCID: PMC4686215
DOI: 10.1007/s40629-015-0046-4

Abstract

Background: ImmunoCAP ISAC 112, is a commercially available molecular allergy IgE multiplex test. Data on the comparison of this rather novel test with extract-based as well as molecular ImmunoCAP singleplex IgE tests is missing.

Objective: To perform a comparison between the ISAC multiplex IgE assay and the ImmunoCAP singleplex test results.

Methods: Serum samples of 101 adults with grass pollen allergy were analysed for sIgE to 112 allergenic molecules represented on the ISAC test as well as to common atopy-related extract-based allergy tests with the ImmunoCAP System (house dust mite [d1], cat [e1], dog [e5], cow's milk [f2], hen's egg [f1], hazelnut [f17], celery [f85], Alternaria alternate [m6], as well as pollen from birch [t3], hazel [t4], mugwort [w6], and ragweed [w1]). Subsequently statistical analysis was performed with the Spearman rank correlation test and the Clopper-Pearson method in order to compare the ISAC multiplex results with the sIgE singleplex results.

Results: The positive percent agreements (PPA) and negative percent agreement (NPA) of corresponding allergens between the ISAC sIgE test and the extract-based singleplex ImmunoCAP results at cutoff 0.1 kUA/l varied between 60-100 % for PPA and 78-97 % for NPA.

Conclusion: When taking into account corresponding allergens molecular testing with the ISAC multiplex test correlates well with ImmunoCAP singleplex results.

Keywords: ISAC; Immuno-CAP; Molecular allergy diagnostics; allergens; component resolved diagnosis; extract-based diagnostics; singleplex multiplex; specific IgE.

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Figures

**Fig. 1. a-h**
Comparison of sIgE results obtained with the ImmunoCAP ISAC test with 8 extract-based ImmunoCAP singelplex tests (n = 101).

See this image and copyright information in PMC

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Comparison of molecular and extract-based allergy diagnostics with multiplex and singleplex analysis

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Comparison of molecular and extract-based allergy diagnostics with multiplex and singleplex analysis

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