Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Jan 15:290:80-3.
doi: 10.1016/j.jneuroim.2015.11.009. Epub 2015 Dec 1.

Pitfalls in the detection of CV2 (CRMP5) antibodies

Lidia Sabater  1 , Albert Saiz  2 , Josep Dalmau  3 , Francesc Graus  4
Affiliations

Pitfalls in the detection of CV2 (CRMP5) antibodies

Lidia Sabater et al. J Neuroimmunol. .

Abstract

CV2 antibodies (CV2-ab) associate with paraneoplastic neurological syndromes (PNS) and small-cell lung cancer. This study was designed to assess the sensitivity of two widely used anti-CV2 commercial kits. Fifty three sera with CV2-ab identified by immunohistochemistry on paraformaldehyde-perfused rat brain were tested with two commercial immunoblot kits (Euroimmun AG, and Ravo Diagnostika) and 4 (7.5%) of them were negative with the commercial kits. The 4 samples were positive by immunofluorescence on HEK293 cells transfected with CRMP5 and immunoblot of these cells lysate. A few CV2-ab-positive sera may be missed by commercial immunoblots. Negative samples from patients with high suspicion for PNS should be tested by alternative methods.

Keywords: CV2 (CRMP5) antibodies; Cancer; Immunoblot; Paraneoplastic.

PubMed Disclaimer

Publication types

LinkOut - more resources