Comparison of enzyme-linked immunosorbent assay (ELISA) with dot hybridization using 32P- or 2-acetylaminofluorene (AAF)-labelled cDNA probes for the detection and characterization of beet necrotic yellow vein virus

Abstract

Beet Necrotic Yellow Vein Virus (BNYVV) was detected by enzyme-linked immunosorbent assay (ELISA) and RNA/DNA dot hybridization using either radiolabelled or non-radioactive probes. Dot hybridization specifically distinguished isolates that could not be distinguished by ELISA. The detection thresholds for ELISA, hybridization with non-radioactive probes and hybridization with radiolabelled probes were 2 ng, 0.2 ng, 0.02 ng of purified virus, respectively. Dot hybridization with non-radioactive probes could be performed on crude infected beet root extracts, thus providing a useful tool for monitoring BNYVV infection and for routine testing in plant breeding programs.