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. 2016 Mar;54(3):802-4.
doi: 10.1128/JCM.03002-15. Epub 2015 Dec 30.

Flow Cytometry To Assess Cerebrospinal Fluid Fungal Burden in Cryptococcal Meningitis

James E Scriven  1 , Lisa M Graham  2 , Charlotte Schutz  3 , Thomas J Scriba  4 , Robert J Wilkinson  5 , David R Boulware  6 , Graeme Meintjes  7 , David G Lalloo  8 , Britta C Urban  9
Affiliations

Flow Cytometry To Assess Cerebrospinal Fluid Fungal Burden in Cryptococcal Meningitis

James E Scriven et al. J Clin Microbiol. 2016 Mar.

Abstract

Fungal burden in the cerebrospinal fluid is an important determinant of mortality in cryptococcal meningitis, but its use in aiding clinical decision making is hampered by the time involved to perform quantitative cultures. Here, we demonstrate the potential of flow cytometry as a novel and rapid technique to address this issue.

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Figures

FIG 1
FIG 1
CSF flow cytometry gating CSF cells (forward scatter [FSC] and side scatter [SCC]), showing a poor definition of cell subsets due to cryptococci (a1), CD45 and LIVE/DEAD stain are used to separate cells (a2), FSC-SSC plot of cryptococci (b), FSC-SSC plot of CSF leukocytes (c), and FSC-SSC plot of dead CD45+ cells.
FIG 2
FIG 2
(a) Scatterplot demonstrating association between flow cytometry counting, and quantitative culture. (b) Bland-Altman plot showing good agreement between flow counting and quantitative culture (gray area indicates the 95% limits of agreement). (c) Association between flow counting and quantitative culture among participants who had not received antifungal therapy before CSF sampling. (d) Bland-Altman plot showing agreement between the two techniques, limited to participants who had not received antifungal therapy before CSF sampling (gray area indicates the 95% limits of agreement).
See this image and copyright information in PMC

References

    1. Park BJ, Wannemuehler KA, Marston BJ, Govender N, Pappas PG, Chiller TM. 2009. Estimation of the current global burden of cryptococcal meningitis among persons living with HIV/AIDS. AIDS 23:525–530. doi: 10.1097/QAD.0b013e328322ffac. - DOI - PubMed
    1. Jarvis JN, Meintjes G, Williams A, Brown Y, Crede T, Harrison TS. 2010. Adult meningitis in a setting of high HIV and TB prevalence: findings from 4961 suspected cases. BMC Infect Dis 10:67. doi: 10.1186/1471-2334-10-67. - DOI - PMC - PubMed
    1. Jarvis JN, Bicanic T, Loyse A, Namarika D, Jackson A, Nussbaum JC, Longley N, Muzoora C, Phulusa J, Taseera K, Kanyembe C, Wilson D, Hosseinipour MC, Brouwer AE, Limmathurotsakul D, White N, van der Horst C, Wood R, Meintjes G, Bradley J, Jaffar S, Harrison T. 2014. Determinants of mortality in a combined cohort of 501 patients with HIV-associated cryptococcal meningitis: implications for improving outcomes. Clin Infect Dis 58:736–745. doi: 10.1093/cid/cit794. - DOI - PMC - PubMed
    1. McMullan BJ, Desmarini D, Djordjevic JT, Chen SC-A, Roper M, Sorrell TC. 2015. Rapid microscopy and use of vital dyes: potential to determine viability of Cryptococcus neoformans in the clinical laboratory. PLoS One 10:e0117186. doi: 10.1371/journal.pone.0117186. - DOI - PMC - PubMed
    1. Brouwer AE, Rajanuwong A, Chierakul W, Griffin GE, Larsen RA, White NJ, Harrison TS. 2004. Combination antifungal therapies for HIV-associated cryptococcal meningitis: a randomised trial. Lancet 363:1764–1767. doi: 10.1016/S0140-6736(04)16301-0. - DOI - PubMed

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