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Review
. 2015 Dec 30;8(1):4.
doi: 10.3390/cancers8010004.

Studies of Tumor Suppressor Genes via Chromosome Engineering

Hiroyuki Kugoh  1   2 , Takahito Ohira  3 , Mitsuo Oshimura  4
Affiliations
Review

Studies of Tumor Suppressor Genes via Chromosome Engineering

Hiroyuki Kugoh et al. Cancers (Basel). .

Abstract

The development and progression of malignant tumors likely result from consecutive accumulation of genetic alterations, including dysfunctional tumor suppressor genes. However, the signaling mechanisms that underlie the development of tumors have not yet been completely elucidated. Discovery of novel tumor-related genes plays a crucial role in our understanding of the development and progression of malignant tumors. Chromosome engineering technology based on microcell-mediated chromosome transfer (MMCT) is an effective approach for identification of tumor suppressor genes. The studies have revealed at least five tumor suppression effects. The discovery of novel tumor suppressor genes provide greater understanding of the complex signaling pathways that underlie the development and progression of malignant tumors. These advances are being exploited to develop targeted drugs and new biological therapies for cancer.

Keywords: PITX1; TERT; chromosome engineering; melanoma; telomerase; tumor suppressor gene.

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Figures

Figure 1
Figure 1
Construction of mouse A9 cells containing a single human chromosome.
Figure 2
Figure 2
Chromosome transfer to cancer cells via MMCT.
Figure 3
Figure 3
Mapping of chromosome regions which coding tumor suppressor genes via the modification of MMCT.
Figure 4
Figure 4
Identification of a functional suppressor gene by combination of MMCT and microarray.
See this image and copyright information in PMC

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