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. 2016 Jan 4;60(3):1924-7.
doi: 10.1128/AAC.01844-15.

Klebsiella pneumoniae Isolate from a New York City Hospital Belonging to Sequence Type 258 and Carrying blaKPC-2 and blaVIM-4

Mariana Castanheira  1 , Lalitagauri M Deshpande  2 , Janet C Mills  2 , Ronald N Jones  3 , Rosemary Soave  4 , Stephen G Jenkins  4 , Audrey N Schuetz  4
Affiliations

Klebsiella pneumoniae Isolate from a New York City Hospital Belonging to Sequence Type 258 and Carrying blaKPC-2 and blaVIM-4

Mariana Castanheira et al. Antimicrob Agents Chemother. .

Abstract

Among 69 of 139 (49.6%) carbapenem-nonsusceptible Enterobacteriaceae carrying blaKPC, 1 Klebsiella pneumoniae was also positive for blaVIM. The isolate belonged to sequence type 258 (ST258) and carried blaKPC-2 on a copy of Tn4401a and blaVIM-4 on a class 1 integron. Genes were located on distinct plasmids belonging to Inc types A/C and FII. Elevated expression of the efflux pump AcrAB-TolC (acrA, 15.3 times) and reduced expression of outer membrane protein genes ompK35 and ompK37 (0.16 and 0.081 times, respectively) associated with various amino acid alterations on OmpK37 were observed. The presence of two carbapenemases in ST258 K. pneumoniae is of great concern due to the ability of this organism to widely disseminate.

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Figures

FIG 1
FIG 1
Schematic representation of the structure carrying blaVIM-4. Genes are represented as open arrows, and the direction of the arrow indicates the transcription orientation. Filled circles, 59-base element (be) recombination sites.
See this image and copyright information in PMC

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