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. 2015 Nov;13(11):715-20.

Association of follicle stimulating hormone receptor promoter with ovarian response in IVF-ET patients

Wang Dan  1 , Gao Jing  1 , Xia Liangbin  1 , Zhang Ting  1 , Zeng Ying  1
Affiliations

Association of follicle stimulating hormone receptor promoter with ovarian response in IVF-ET patients

Wang Dan et al. Iran J Reprod Med. 2015 Nov.

Abstract

Background: Poor ovarian response phenomenon has been observed in some of the in vitro fertilization-embryo transfer patients. Some investigations found that follicle stimulating hormone receptor ( FSHR ) gene plays a role in the process, but no direct evidence shows the correlation between genotypes of FSHR and ovarian response.

Objective: Exploring the molecular mechanism behind the mutation of FSH R promoter association with ovarian granulosa cells and poor ovarian response.

Materials and methods: This cross sectional study was performed using 158 women undergoing the controlled short program ovarian stimulation for IVF treatment. The 263 bp DNA fragments before the follicle stimulating hormone (FSH) receptor 5' initiation site were sequenced in the patients under IVF cycle, 70 of which had poor ovarian response and 88 showed normal ovarian responses.

Results: With a mutation rate of 40%, 63 in 158 cases showed a 29(th) site G→A point mutation; among the mutated cases, the mutation rate of the poor ovarian responders was significantly higher than the normal group (60% versus 23.9%; χ2=21.450, p<0.001). Besides, the variability was also obvious in antral follicle count, and ovum pick-ups. The estradiol peak values and the number of mature eggs between the two groups had significant difference. However, there was no obvious variability (t=0.457, p=0.324) in the basic FSH values between the two groups (normal group, 7.2±2.3 U/L; mutation group, 7.1±2.0 U/L).

Conclusion: The activity of FSHR promoter is significantly affected by the 29(th) site G→A mutation that will weaken promoter activity and result in poor response to FSH.

Keywords: Follicle stimulating hormone receptor; Infertility; Ovarian granulosa cells; Ovarian response; Promoter.

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Figures

Figure 1
Figure 1
The electrophoresis of the PCR product of the FSHR promoter region from the genomic DNA of each group
Figure 2
Figure 2
The graph of a homozygous mutation in the FSHR sequence (Sample 11)
Figure 3
Figure 3
The graph of a heterozygous mutation in the FSHR sequence (Sample 16)
See this image and copyright information in PMC

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