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. 1989 Aug 30;163(1):194-200.
doi: 10.1016/0006-291x(89)92120-7.

Proalbumin is processed to serum albumin in COS-1 cells transfected with cDNA for rat albumin

K Oda  1 , N Takami, T Fujiwara, Y Misumi, Y Ikehara
Affiliations

Proalbumin is processed to serum albumin in COS-1 cells transfected with cDNA for rat albumin

K Oda et al. Biochem Biophys Res Commun. .

Abstract

Synthesis and processing of rat albumin were investigated in COS-1 cells transiently expressing rat albumin. Analysis using isoelectric focusing revealed that serum-type albumin, which is indistinguishable from the counterpart isolated from rat hepatocyte cuture medium, was secreted from the transfected COS-1 cells, indicating that proalbumin is effectively converted into serum albumin in the COS-1 cells, if not completely. Furthermore methylamine was found to cause the diminution of serum albumin released from the cells, substantiating that the proteolytical conversion of proalbumin occurs in the Golgi complex before discharge from the COS-1 cells.

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