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. 2015 Nov;42(6):361-4.
doi: 10.1159/000440821. Epub 2015 Nov 3.

14 Years of Polish Experience in Non-Invasive Prenatal Blood Group Diagnosis

Agnieszka Orzińska  1 , Katarzyna Guz  1 , Marzena Dębska  2 , Małgorzata Uhrynowska  1 , Zbigniew Celewicz  3 , Mirosław Wielgo  4 , Ewa Brojer  1
Affiliations

14 Years of Polish Experience in Non-Invasive Prenatal Blood Group Diagnosis

Agnieszka Orzińska et al. Transfus Med Hemother. 2015 Nov.

Erratum in

  • Erratum.
    [No authors listed] [No authors listed] Transfus Med Hemother. 2016 Jan;43(1):54. doi: 10.1159/000443001. Epub 2016 Feb 9. Transfus Med Hemother. 2016. PMID: 27022324 Free PMC article.

Abstract

Background: Blood cell antigens may cause maternal alloimmunization leading to fetal/newborn disorders. Non-invasive prenatal diagnostics (NIPD) of the blood group permits the determination of feto-maternal incompatibility.

Aim: To evaluate 14 years of blood group NIPD at the Institute of Hematology and Transfusion Medicine (IHTM) in Warsaw.

Methods: Plasma DNA from 536 RhD-negative, 24 Rhc-negative, 26 RhE-negative, 43 K-negative, and 42 HPA-1a-negative pregnant women was examined by real-time PCR to detect RHD, RHCE*c, RHCE*E, RHCE*C, KEL*01 and HPA*1A, respectively. We tested for CCR5, SRY or bi-allelic polymorphisms and quantified the total or fetal DNA.

Results: The results of fetal antigen status prediction by NIPD in all but one case (false-positive result of KEL*01) were correct taking neonate serology as a reference. It was confirmed that all negative results of NIPD contained fetal DNA except for four cases where there was no difference between the parents' polymorphisms.

Conclusions: A fetal genotype compatible with the mother was determined in 25% of all pregnancies tested at the IHTM for the fetal blood group. These cases were not at risk of disease, so it was possible to avoid invasive procedures.

Keywords: Alloimmunization; Non-invasive prenatal diagnostics; Real-time PCR.

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