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Comment
. 2016 Jan;22(1):16-7.
doi: 10.1038/nm.4019.

Suppressive activity of human regulatory T cells is maintained in the presence of TNF

Bruno Zaragoza  1 , Xin Chen  2   3   4 , Joost J Oppenheim  3 , Audrey Baeyens  1 , Sylvie Gregoire  1 , Driss Chader  1 , Guy Gorochov  1   5 , Makoto Miyara  1   5 , Benoît L Salomon  1
Affiliations
Comment

Suppressive activity of human regulatory T cells is maintained in the presence of TNF

Bruno Zaragoza et al. Nat Med. 2016 Jan.
No abstract available

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Conflict of interest statement

COMPETING FINANCIAL INTERESTS

The authors declare no competing financial interests.

Figures

Figure 1
Figure 1
Human Treg cell suppressive activity is not impaired in the presence of TNF. (a,b) Suppressive activity of purified Treg cells co-cultured with carboxyfluorescein succinimidyl ester (CFSE)-labeled Teff cells was measured at day 4 using an APC and plate-bound CD3-specific antibody assay (a) or a bead assay (b) in the presence (+ TNF) or absence of TNF. Representative CFSE profile of Teff cells at a 1:1 Teff:Treg ratio (left) and mean ± s.d. of % of suppression at different Teff:Treg cell ratios (right) from 7 (a) and 8 (b) independent experiments using different donors for each experiment. (c,d) CD25 and FOXP3 expression on Treg cells that were cultivated alone with high-dose IL-2 (c) or increasing doses of IL-2 (d) in the presence (+ TNF) or absence of TNF for 3 d. (c) One representative experiment of 17–20 different donors (left) and cumulative data (17 donors for the CD25 staining and 20 donors for the FOXP3 staining) of mean fluorescence intensity (MFI) of FOXP3 and CD25 (right). (d) MFI ± s.d. of FOXP3 and CD25 at different IL-2 doses from two independent responses using two different donors. The arrow shows values before culture. (e,f) Treg cells were first cultivated with high-dose IL-2 in the presence (+ TNF) or absence of TNF for 3 d. After this, they were washed twice to remove cytokines and then analyzed for suppressive activity at day 4 in the absence of TNF. Representative CFSE profile of Teff cells at a 1:1 Teff:Treg ratio (left) and ± s.d. of the percentage of suppression at different Teff:Treg cell ratios (right) using an APC- and plate-bound CD3-specific antibody assay (e, five different donors) or a bead assay (f, three different donors). *P < 0.05, **P < 0.01, ****P < 0.0001, obtained using nonparametric one-sided Wilcoxon signed-rank tests.
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References

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