Arsenic-induced Histological Alterations in Various Organs of Mice

Abstract

Deposition of arsenic in mice through groundwater is well documented but little is known about the histological changes of organs by the metalloid. Present study was designed to evaluate arsenic-induced histological alterations in kidney, liver, thoracic artery and brain of mice which are not well documented yet. Swiss albino male mice were divided into 2 groups and treated as follows: Group 1: control, 2: arsenic (sodium arsenite at 10 mg/kg b.w. orally for 8 wks). Group 2 showed marked degenerative changes in kidney, liver, thoracic artery, and brain whereas Group 1 did not reveal any abnormalities on histopathology. We therefore concluded that arsenic induces histological alterations in the tested organs.

Keywords: Arsenic; Histopathology; Mice organs.

Figure 1

A) Photomicrograph of kidney showing normal architecture H&E X200 Group 1, B) Photomicrograph of kidney showing fat bodies, tubular degeneration, intratubular degeneration, and congestion, H&E 200X Group 2.

Figure 2

A). Photomicrograph of liver showing normal architecture H&E 200X Group 1, B). Photomicrograph of liver showing necrosis, H&E 200X Group 2.

Figure 3

A). Photomicrograph of thoracic artery showing normal architecture H&E 200X Group 1, B). Photomicrograph of thoracic artery showing capillary wall was destructed, H&E 200X Group 2.

Figure 4

A). Photomicrograph of brain showing normal architecture H&E X200 Group 1, B). Photomicrograph of brain showing edema, intracellular space, edematous change, H&E 200X Group 2.

Figure 5

Comparison of serum glutamate-pyruvate transaminase (SGPT) levels (mean ± SE) between control and arsenic-treated mice. Blank and black bar represent the control and arsenic-treated mice, respectively. *Significantly different from control group at p<0.01. p value was from Independent sample t-test.

Figure 6

Comparison of blood urea nitrogen (BUN) levels (mean ± SE) between control and arsenic-treated mice. Blank and black bar represent the control and arsenic-treated mice, respectively. *Significantly different from control group at p<0.05. p value was from Independent sample t-test.