Expression of human and viral ets genes in E. coli production of human ets-2-specific monoclonal antibodies

Abstract

Full-length and deletion variants of the ets proto-oncogene and v-ets oncogene of E26 virus were expressed in E. coli using the previously described expression vectors pANH1, pJL6 and pJLA16. The recombinant proteins were expressed at greater than 5% of the total cellular proteins and were characterized by Western blot analysis using ets-specific antipeptide antibodies. A number of monoclonal antibodies were raised against p35, the expressed product of the partial human ets-2 proto-oncogene construct. These monoclonal antibodies are highly specific and recognize the p56 ets-2 product from several human cell lines.