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. 2016 Apr;5(2):307-22.
doi: 10.1002/mbo3.329. Epub 2016 Jan 8.

Microvirga massiliensis sp. nov., the human commensal with the largest genome

Aurélia Caputo  1 , Jean-Christophe Lagier  1 , Saïd Azza  1 , Catherine Robert  1 , Donia Mouelhi  1 , Pierre-Edouard Fournier  1 , Didier Raoult  1   2
Affiliations

Microvirga massiliensis sp. nov., the human commensal with the largest genome

Aurélia Caputo et al. Microbiologyopen. 2016 Apr.

Abstract

Microvirga massiliensis sp. nov. strain JC119(T) is a bacteria isolated in Marseille from a stool sample collected in Senegal. The 16S rRNA (JF824802) of M. massiliensis JC119(T) revealed 95% sequence identity with Microvirga lotononidis WSM3557(T) (HM362432). This bacterium is aerobic, gram negative, catalase positive, and oxidase negative. The draft genome of M. massiliensis JC119(T) comprises a 9,207,211-bp-long genome that is the largest bacterial genome of an isolate in humans. The genome exhibits a G+C content of 63.28% and contains 8685 protein-coding genes and 77 RNA genes, including 21 rRNA genes. Here, we describe the features of M. massiliensis JC119(T), together with the genome sequence information and its annotation.

Keywords: Culturomics; Microvirga massiliensis; large genome; taxonogenomics.

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Figures

Figure 1
Figure 1
(A) Reference mass spectrum from Microvirga massiliensis JC119T. (B) Gel view comparing M. massiliensis strain to other Methylobacteriaceae family. The gel view displays the raw spectra of loaded spectrum files arranged in a pseudo‐gel‐like look. The x‐axis records the m/z value. The left y‐axis displays the running spectrum number originating from subsequent spectra loading. The peak intensity is expressed by a Gray scale scheme code. The color bar and the right y‐axis indicate the relation between the color a peak is displayed with and the peak intensity in arbitrary units. Displayed species are indicated on the left.
Figure 2
Figure 2
(A) Gram straining of Microvirga massiliensis JC119T. (B) Transmission electron microscopy of M. massiliensis JC119T, taken using a Morgagni 268D (Philips Amsterdam, Netherlands) at an operating voltage of 60 kV. The scale represents 500 nm.
Figure 3
Figure 3
Phylogenetic tree highlighting the position of Microvirga massiliensis JC119T relative to other type strains within the Microvirga genus and closely related species. The strains and their corresponding accession number for 16S rRNA genes are indicated in parentheses. Cupriavidus taiwanensis strain LMG 19424T was used as outgroup. The scale bar represents a 2% nucleotide sequence divergence.
Figure 4
Figure 4
Circular representation of the Microvirga massiliensis strain JC119T genome. From outside to the center: contigs (red/gray), COG category of genes on the forward strand (three circles), genes on forward strand (blue circle), genes on the reverse strand (red circle), COG category on the reverse strand (three circles), GC content.
Figure 5
Figure 5
Distribution of functional classes of predicted genes in the genomes from Microvirga massiliensis, Microvirga aerilata, Microvirga flocculans, Microvirga lotononidis, and Microvirga lupini chromosomes, according to the COG category.
See this image and copyright information in PMC

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