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. 2016 Mar;13(3):233-6.
doi: 10.1038/nmeth.3727. Epub 2016 Jan 11.

Predictable tuning of protein expression in bacteria

Mads T Bonde  1 , Margit Pedersen  1 , Michael S Klausen  1 , Sheila I Jensen  1 , Tune Wulff  1 , Scott Harrison  1 , Alex T Nielsen  1 , Markus J Herrgård  1 , Morten O A Sommer  1
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Free article

Predictable tuning of protein expression in bacteria

Mads T Bonde et al. Nat Methods. 2016 Mar.
Free article

Abstract

We comprehensively assessed the contribution of the Shine-Dalgarno sequence to protein expression and used the data to develop EMOPEC (Empirical Model and Oligos for Protein Expression Changes; http://emopec.biosustain.dtu.dk). EMOPEC is a free tool that makes it possible to modulate the expression level of any Escherichia coli gene by changing only a few bases. Measured protein levels for 91% of our designed sequences were within twofold of the desired target level.

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