Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity
- PMID: 26752769
- PMCID: PMC4770512
- DOI: 10.1038/nmeth.3728
Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity
Abstract
We report scM&T-seq, a method for parallel single-cell genome-wide methylome and transcriptome sequencing that allows for the discovery of associations between transcriptional and epigenetic variation. Profiling of 61 mouse embryonic stem cells confirmed known links between DNA methylation and transcription. Notably, the method revealed previously unrecognized associations between heterogeneously methylated distal regulatory elements and transcription of key pluripotency genes.
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Comment in
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Epigenomics: Parallel single-cell sequencing.Nat Rev Genet. 2016 Mar;17(3):125. doi: 10.1038/nrg.2016.5. Epub 2016 Jan 25. Nat Rev Genet. 2016. PMID: 26806414 No abstract available.
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