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. 2015 Sep 9;9(4):565-77.
doi: 10.3897/CompCytogen.v9i4.5741. eCollection 2015.

Cytogenetic description of the earthworm Drawida ghilarovi Gates, 1969 (Oligochaeta, Moniligastridae) from the southern Russian Far East

Alim P Anisimov  1 , Galina V Roslik  2 , Gennady N Ganin  3
Affiliations

Cytogenetic description of the earthworm Drawida ghilarovi Gates, 1969 (Oligochaeta, Moniligastridae) from the southern Russian Far East

Alim P Anisimov et al. Comp Cytogenet. .

Abstract

Sixty-six specimens of the earthworm Drawida ghilarovi Gates, 1969 (Oligochaeta, Moniligastridae) from 15 localities of the southern Russian Far East were studied cytogenetically. We examined chromosome sets during mitosis and diakinesis as well as DNA content in the spermatogenous and somatic cell nuclei. The populations and morphs displayed no differences in karyotype and ploidy levels estimated in terms of both chromosome number and DNA mass index: n = 10, 2n = 20; c = 1.1 pg, 2c = 2.2 pg. We conclude that polyploidy as a species- or race-forming factor is not typical of these earthworms.

Keywords: Drawida; Karyotype; Moniligastridae; Oligochaeta; chromosomal set; earthworm; nuclear DNA content.

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Figures

Figure 1.
Figure 1.
Map showing collection sites (1–15, see Table 1) of different morphs (I-III) of the tropical worm Drawida ghilarovi in the southern Russian Far East. I – black, black-reddish; II – brownish, yellow-brownish; III – aquamarine, bluish-grey, grey, greenish-grey, brownish-blue morphs.
Figure 2.
Figure 2.
Distribution of nuclear DNA content in spermatogenous and somatic cells from seminal vesicles of Drawida ghilarovi sampled in the Kedrovaya Pad’ Nature Reserve. Abscissa, DNA content (conventional units and haploid “c” units). Ordinate, number of nuclei.
Figure 3.
Figure 3.
Cluster of synchronously dividing spermatogonia from a seminal vesicle of Drawida ghilarovi sampled in the Lasovsky Nature Reserve. Arrows point at two tetraploid (4n8c) mitoses among ordinary diploid ones (2n4c). Scale bar: 5 µm.
Figure 4.
Figure 4.
Mitotic metaphase (a) and karyogram (b) of Drawida ghilarovi from the Sikhote-Alin Nature Reserve. 2n = 20. Scale bars: 5 µm.
Figure 5.
Figure 5.
Early diakinesis (a) and meiotic metaphase-I (b) in Drawida ghilarovi from the Kedrovaya Pad’ Nature Reserve. n = 10. Scale bars: 5 µm.
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References

    1. Anisimov AP. (1992) Simple method of preparing permanent squash preparations using cellophane. Tsitologiya 34(11/12): 110–112. [In Russian] - PubMed
    1. Anisimov AP. (2005) Endopolyploidy as a morphogenetic factor of development. Cell Biology International 29: 993–1004. doi: 10.1016/j.cellbi.2005.10.013 - DOI - PubMed
    1. Bakhtadze NG, Bakhtadze GI, Kvavadze ESh. (2008) The chromosome numbers of Georgian earthworms (Oligochaeta: Lumbricidae). Comparative Cytogenetics 2(1): 79–83.
    1. Bancroft JD, Cook HC. (1999) Proteins and nucleic acids. In: Theory and Practice of Histological techniques. Edinburg, London, New York, Philadelphia, St Louis, Sydney, Toronto, 139–150.
    1. Blakemore RJ. (2003) Japanese earthworms (Annelida: Oligochaeta): a review and checklist of species. Organisms, Diversity and Evolution 3(3): 241–244. doi: 10.1078/1439-6092-00082 - DOI

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