Human fallopian tube proteome shows high coverage of mesenchymal stem cells associated proteins

Abstract

The object of this research was to report a draft proteome of human fallopian tube (hFT) comprises 5416 identified proteins, which could be considered as a physiological reference to complement Human Proteome Draft. The proteomic raw data and metadata were stored in an integrated proteome resources centre iProX (IPX00034300). This hFT proteome contains many hFT markers newly identified by mass spectrum. This hFT proteome comprises 660 high-, 3605 medium- and 1181 low-abundant proteins. Ribosome, cytoskeleton, vesicle and protein folding associated proteins showed obvious tendency to be higher abundance in hFT. The extraordinary high coverage of mesenchymal stem cells (MSCs)-associated proteins were identified in this hFT proteome, which highly supported that hFT should contain a plenty of MSCs.

Keywords: human fallopian tube; mesenchymal stem cell; oviduct; proteome.

Figure 1. Status of hFT proteome and its overlap of Rungruang data

(A) Two replicates were with 5177 and 3712 identified proteins respectively, totally sum up to 5416, and with 3473 overlapped. (B) Boxplots of original and median normalized LFQ values of the two replicates showed no significant difference. The median of rep. 1 and 2 were 1045 and 989 respectively. (C) Abundance distributions of the high-, medium- and low-abundant proteins of the whole hFT proteome and its two replicates. (D) The overlap of our hFT proteome and Rungruang data is merely 1233 proteins, showed high complementary of the two datasets. *The number of identified proteins was slightly more than individual statistics in both datasets, due to split of a protein group (count 1) into its members (count >1) that all were identified in the other dataset.