Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Aug;8(8):2411-5.
doi: 10.1002/j.1460-2075.1989.tb08371.x.

In vitro reconstitution of anticodon nuclease from components encoded by phage T4 and Escherichia coli CTr5X

M Amitsur  1 , I Morad, G Kaufmann
Affiliations

In vitro reconstitution of anticodon nuclease from components encoded by phage T4 and Escherichia coli CTr5X

M Amitsur et al. EMBO J. 1989 Aug.

Abstract

During phage T4 infection of Escherichia coli strains containing the prr locus the host tRNALys undergoes cleavage-ligation in reactions catalyzed by anticodon nuclease, polynucleotide kinase and RNA ligase. Known genetic determinants of anticodon nuclease are prr, which restricts T4 mutants lacking polynucleotide kinase or RNA ligase, and stp, the T4 suppressor of prr encoded restriction. The present communication describes an in vitro anticodon nuclease assay in which the specific cleavage of tRNALys is driven by an extract from E. coli prrr (restrictive) cells infected by phage T4. The in vitro anticodon nuclease reaction requires factor(s) encoded by prr, is stimulated by a synthetic Stp polypeptide and appears to require additional T4 induced factor(s) distinct from Stp.

PubMed Disclaimer

References

    1. J Virol. 1974 Apr;13(4):888-97 - PubMed
    1. Virology. 1975 Mar;64(1):144-5 - PubMed
    1. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3098-102 - PubMed
    1. J Mol Biol. 1978 Aug 5;123(2):221-33 - PubMed
    1. J Mol Biol. 1982 Jan 15;154(2):273-86 - PubMed

Publication types