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. 2017 Aug;25(8):2494-2501.
doi: 10.1007/s00167-016-3984-6. Epub 2016 Jan 14.

One-step surgery with multipotent stem cells and Hyaluronan-based scaffold for the treatment of full-thickness chondral defects of the knee in patients older than 45 years

Alberto Gobbi  1 , Celeste Scotti  2 , Georgios Karnatzikos  3 , Abhishek Mudhigere  3 , Marc Castro  4 , Giuseppe M Peretti  2   5
Affiliations

One-step surgery with multipotent stem cells and Hyaluronan-based scaffold for the treatment of full-thickness chondral defects of the knee in patients older than 45 years

Alberto Gobbi et al. Knee Surg Sports Traumatol Arthrosc. 2017 Aug.

Erratum in

Abstract

Purpose: The aim of this study is to prospectively evaluate the medium-term effectiveness and regenerative capability of autologous adult mesenchymal stem cells, harvested as bone marrow aspirate concentrate (BMAC), along with a hyaluronan-based scaffold (Hyalofast) in the treatment of ICRS grade 4 chondral lesions of the knee joint, in patients older than 45 years.

Methods: A study group of 20 patients with an age >45 years (mean 50.0 ± 4.1 years) was compared to a control group of 20 patients with an age <45 years (mean 36.6 ± 5.0). Patients were prospectively evaluated for 4 years. All patients were evaluated with MRI, KOOS, IKDC, VAS and Tegner scores preoperatively and at two-year and final follow-up.

Results: At final follow-up, all scores significantly improved (P < 0.001) as follows: all KOOS score categories; Tegner 2 (range 0-4) to 6 (range 4-8) and 3 (range 0-6) to 6 (range 3-10); IKDC subjective (39.2 ± 16.5 to 82.2 ± 8.9) and (40.8 ± 13.9 to 79.4 ± 14.6), in the study and control group respectively. In addition, we show that results are affected by lesion size and number but not from concomitant surgical procedures. MRI showed complete filling in 80 % of patients in the study group and 71 % of patients in the control group. Histological analysis conducted in three patients from the study and two patients from the control group revealed good tissue repair with a variable amount of hyaline-like tissue.

Conclusion: Treatment of cartilage lesions with BMAC and Hyalofast is a viable and effective option that is mainly affected by lesion size and number and not by age. In particular, it allows to address the >45 years population with functional outcomes that are comparable to younger patients at final follow-up.

Level of evidence: Prospective cohort study, Level II.

Keywords: Bone Marrow Aspirate Concentrate (BMAC); Cartilage; Cartilage lesion; Hyaluronan; Mesenchymal Stem cells (MSCs); Scaffold.

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Conflict of interest statement

Dr. Gobbi is Scientific Consultant for Anika Therapeutics Inc. Massachusetts, USA.

Figures

Fig. 1
Fig. 1
a Sagittal section magnetic resonance imaging of a grade 4 chondral lesion involving articular surface of medial femoral condyle in 50-year-old male. b 1-year follow-up MRI showing complete filling of the defect. c 5-year follow-up MRI showing establishment of smooth articular surface
Fig. 2
Fig. 2
Second look arthroscopy view at 1-year follow-up of grade 4 patellar chondral lesion showing filling of the defect with a well-integrated, smooth surfaced and stable regenerated cartilage
Fig. 3
Fig. 3
Biopsy report at 2-year follow up. a Safranin O staining shows hyaline-like tissue, intensely stained for proteoglycans, slightly hypercellular and with some fibrous features. The superficial layer is regular, the surface is smooth and the cells are homogeneously distributed. The subchondral bone is normal and normal passage bone/cartilage. b Collagen type I immunostaining showing no collagen type I positive matrix. c Collagen Type II immunostaining showing presence of type II collagen within the matrix
See this image and copyright information in PMC

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