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. 2016:2016:2650849.
doi: 10.1155/2016/2650849. Epub 2015 Dec 6.

Effect of Periodic Granulocyte Colony-Stimulating Factor Administration on Endothelial Progenitor Cells and Different Monocyte Subsets in Pediatric Patients with Muscular Dystrophies

Andrzej Eljaszewicz  1 , Dorota Sienkiewicz  2 , Kamil Grubczak  3 , Bożena Okurowska-Zawada  2 , Grażyna Paszko-Patej  2 , Paula Miklasz  1 , Paulina Singh  1 , Urszula Radzikowska  1 , Wojciech Kulak  2 , Marcin Moniuszko  4
Affiliations

Effect of Periodic Granulocyte Colony-Stimulating Factor Administration on Endothelial Progenitor Cells and Different Monocyte Subsets in Pediatric Patients with Muscular Dystrophies

Andrzej Eljaszewicz et al. Stem Cells Int. 2016.

Abstract

Muscular dystrophies (MD) are heterogeneous group of diseases characterized by progressive muscle dysfunction. There is a large body of evidence indicating that angiogenesis is impaired in muscles of MD patients. Therefore, induction of dystrophic muscle revascularization should become a novel approach aimed at diminishing the extent of myocyte damage. Recently, we and others demonstrated that administration of granulocyte colony-stimulating factor (G-CSF) resulted in clinical improvement of patients with neuromuscular disorders. To date, however, the exact mechanisms underlying these beneficial effects of G-CSF have not been fully understood. Here we used flow cytometry to quantitate numbers of CD34+ cells, endothelial progenitor cells, and different monocyte subsets in peripheral blood of pediatric MD patients treated with repetitive courses of G-CSF administration. We showed that repetitive cycles of G-CSF administration induced efficient mobilization of above-mentioned cells including cells with proangiogenic potential. These findings contribute to better understanding the beneficial clinical effects of G-CSF in pediatric MD patients.

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Figures

Figure 1
Figure 1
The summary of analyses of changes in CD34+ cells numbers after (a) course 1, (b) course 2, and (c) course 3 of G-CSF administration in MD pediatric patients.
Figure 2
Figure 2
The summary of analyses of EPC numbers (expressing CD34+CD133+CD309+ phenotype) in MD pediatric individuals after (a) course 1, (b) course 2, and (c) course 3 of G-CSF administration.
Figure 3
Figure 3
Time course changes in Ang-1 (upper row) and Ang-2 (bottom row) plasma levels in pediatric patients with MD after (a) course 1, (b) course 2, and (c) course 3 of G-CSF administration.
Figure 4
Figure 4
Effect of (a) course 1, (b) course 2, and (c) course 3 of G-CSF administration on absolute numbers of CD14++CD16− (upper row), CD14++CD16+ (middle row), and CD14+CD16++ (bottom row) monocytes in pediatric patients with MD.
Figure 5
Figure 5
Time course changes in sCD163 plasma levels after (a) course 1, (b) course 2, and (c) course 3 of G-CSF administration in MD pediatric patients.
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References

    1. Emery A. E. H. The muscular dystrophies. The Lancet. 2002;359(9307):687–695. doi: 10.1016/s0140-6736(02)07815-7. - DOI - PubMed
    1. Mercuri E., Muntoni F. Muscular dystrophies. The Lancet. 2013;381(9869):845–860. doi: 10.1016/s0140-6736(12)61897-2. - DOI - PubMed
    1. Meilleur K. G., Jain M. S., Hynan L. S., et al. Results of a two-year pilot study of clinical outcome measures in collagen VI- and laminin alpha2-related congenital muscular dystrophies. Neuromuscular Disorders. 2015;25(1):43–54. doi: 10.1016/j.nmd.2014.09.010. - DOI - PMC - PubMed
    1. Kuhr C. S., Lupu M., Storb R. Hematopoietic cell transplantation directly into dystrophic muscle fails to reconstitute satellite cells and myofibers. Biology of Blood and Marrow Transplantation. 2007;13(8):886–888. doi: 10.1016/j.bbmt.2007.04.012. - DOI - PMC - PubMed
    1. McGreevy J. W., Hakim C. H., McIntosh M. A., Duan D. Animal models of Duchenne muscular dystrophy: from basic mechanisms to gene therapy. Disease Models & Mechanisms. 2015;8(3):195–213. doi: 10.1242/dmm.018424. - DOI - PMC - PubMed

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