FOXO3a Gene Polymorphism Associated with Asthma in Indian Population

Abstract

Asthma is a chronic inflammatory disorder delineated by a heightened immunological response due to environmental or genetic factors. Single nucleotide polymorphism studies have shown that FOXO3a plays a pivotal role in maintaining immunoregulation. Polymorphism in FOXO3a has been linked to inflammatory diseases such as chronic obstructive pulmonary disease (COPD), Rheumatoid Arthritis, and Crohn's disease suggesting that FOXO3a may be associated with asthma. Airway inflammation in asthma is characterized by activation of T helper type 2 (Th2) T cells and Foxo family members are reported to play critical roles in the suppression of T cell activation. Thus this study was undertaken to investigate an association between single nucleotide polymorphism of the FOXO3a (rs13217795, C>T transition) gene and asthma in Indian population. To our knowledge we are the first ones reporting an association between FOXO3a and asthma.

Figure 1

Gel representing typical restriction fragment length polymorphism patterns obtained by subjecting PCR products to restriction digestion by enzyme, PagI (New England Biolabs Inc., USA). Lane 1 consists of the 100 bp DNA marker (Bangalore Genei, India). Lanes 2 and 3 represent RFLP pattern for homozygous mutant alleles (TT). Lanes 4 and 5 represent RFLP pattern for heterozygous (CT) alleles. Lanes 6 and 7 represent RFLP pattern for homozygous wild type (CC) alleles.