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. 2016 Jan 20:10.1007/7651_2015_299.
doi: 10.1007/7651_2015_299. Online ahead of print.

Effects of Malignant Melanoma Initiating Cells on T-Cell Activation

Tobias Schatton  1   2 , Ute Schütte  2   3 , Markus H Frank  4   5
Affiliations

Effects of Malignant Melanoma Initiating Cells on T-Cell Activation

Tobias Schatton et al. Methods Mol Biol. .

Abstract

Although human malignant melanoma is a highly immunogenic cancer, both the endogenous antitumor immune response and melanoma immunotherapy often fail to control neoplastic progression. Accordingly, characterizing melanoma cell subsets capable of evading antitumor immunity could unravel optimized treatment strategies that might reduce morbidity and mortality from melanoma. By virtue of their preferential capacity to modulate antitumor immune responses and drive inexorable tumor growth and progression, malignant melanoma-initiating cells (MMICs) warrant closer investigation to further elucidate the cellular and molecular mechanisms underlying melanoma immune evasion and immunotherapy resistance. Here we describe methodologies that enable the characterization of immunoregulatory effects of purified MMICs versus melanoma bulk populations in coculture with syngeneic or allogeneic lymphocytes, using [3H]thymidine incorporation, enzyme-linked immunosorbent spot (ELISPOT), or ELISA assays. These assays were traditionally developed to analyze alloimmune processes and we successfully adapted them for the study of tumor-mediated immunomodulatory functions.

Keywords: Antitumor immunity; Cancer stem cell; Cytokines; ELISA; ELISPOT; Immune escape; Immunology; Immunomodulation; Immunoregulatory; Melanoma; PBMC; Proliferation; T cell activation; T effector cells; Tumor initiating cell; [3H]thymidine incorporation.

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