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. 2016 Jan 26;113(4):801-5.
doi: 10.1073/pnas.1525084113. Epub 2016 Jan 19.

Discovery of RNA splicing and genes in pieces

Arnold J Berk  1
Affiliations

Discovery of RNA splicing and genes in pieces

Arnold J Berk. Proc Natl Acad Sci U S A. .
No abstract available

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Conflict of interest statement

The author declares no conflict of interest.

Figures

Fig. 1.
Fig. 1.
Single-stranded RNA tails observed at both 5′ and 3′ ends of R-loops of Ad2 hexon mRNA and the Ad2 HindIII A fragment. (Upper) R-loop formation near the temperature of DNA melting in 70% formamide, 0.4 M NaCl. Arrowheads indicate 3′ ends of strands. Adapted from ref. . (Lower) R-loop in Ad2 HindIII A restriction fragment formed with purified hexon mRNA. (A) EM of R-loop showing all of the Hind III A DNA fragment with an R-loop at the lower end. (B) Higher magnification of the R-loop. In A and B, the upper arrow points to a single-stranded region at the 3′ end of the RNA, and the bottom arrow points to a single-stranded region at the 5′ end. In these formamide spreads, single-stranded DNA appears thinner and has a more irregular path than double-stranded DNA or RNA-DNA hybrid, which appear similar. (C) Interpretation of EM in B. The hexon mRNA is represented in red and each strand of the Hind III A DNA in black. Regions where the red RNA is parallel to the black DNA strand represent base paired regions of RNA-DNA hybrid. Regions where the two black DNA strands are parallel represent DNA-DNA duplex. A, B, and C adapted from ref. .
Fig. 2.
Fig. 2.
EM of hybrid between purified hexon mRNA and the transcribed strand of the Ad2 EcoRI A DNA fragment. (A) Diagram of the positions of hexon mRNA exons (red) and the introns between them (A, B, and C in light blue) in the left ∼25 kb of the Ad2 genome. (B) EM of hybrid between hexon mRNA and the EcoRI A coding strand. In the interpretation shown on the right, the mRNA is shown in red and DNA in black. Regions where the red RNA is parallel to the black DNA strand represent base paired regions of RNA-DNA hybrid. Adapted from ref. .
See this image and copyright information in PMC

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