doi: 10.1073/pnas.74.6.2259.
Synthesis of opiate peptides by a clonal pituitary tumor cell line
- PMID: 267923
- PMCID: PMC432149
- DOI: 10.1073/pnas.74.6.2259
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Synthesis of opiate peptides by a clonal pituitary tumor cell line
Proc Natl Acad Sci U S A.
1977 Jun.
Abstract
Clonal mouse pituitary tumor cells, AtT-20, synthesize at least four species of peptides with opiate activity. The endorphin concentration of AtT-20 cells was estimated to be 300-600 pmol/mg of protein. The two most abundant endorphins with apparent molecular weights of 1800 and 2400 were purified 300- and 24-fold, respectively; additional minor components were found with apparent molecular weights of greater than 3000 and less than 750.
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