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. 2016 Jan 22:6:19651.
doi: 10.1038/srep19651.

Two Genetic Variations in the IRF8 region are associated with Behçet's disease in Han Chinese

Yanni Jiang  1 , Hong Wang  2 , Hongsong Yu  1 , Lin Li  1 , Dengfeng Xu  1 , Shengping Hou  1 , Aize Kijlstra  3 , Peizeng Yang  1
Affiliations

Two Genetic Variations in the IRF8 region are associated with Behçet's disease in Han Chinese

Yanni Jiang et al. Sci Rep. .

Abstract

Several modulatory factors in the TLR signaling pathway including IRF3, IRF7, IRF8, TRIM20, MYD88 and NF-κB1 have been associated with autoimmune disease. In this study, we investigated the association of 13 SNPs for these genes with Behçet's disease (BD) and Vogt-Koyanagi-Harada (VKH) syndrome using a polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assay. Haplotype and linkage disequilibrium (LD) analysis were performed by Haploview4.2. IRF8 mRNA expression and cytokine production was tested by real-time PCR and ELISA. Two SNPs near IRF8 were associated with BD (for rs17445836 GG genotype, Pc = 9.56 × 10(-8), OR = 2.044; for rs11642873 AA genotype, Pc = 9.24 × 10(-7), OR = 1.776). No significant association was found for the 13 SNPs tested with VKH syndrome. Haplotype analysis of the two positive SNPs revealed that the AG haplotype was significantly increased in BD patients (Pc = 2.60 × 10(-8), OR = 1.646). Functional studies revealed an increased mRNA expression of IRF8 and IFN-γ production and a decreased production of IL-10 in rs17445836 carriers with the GG genotype. Increased expression of IRF8 as well as IFN-γ production and a decreased production of IL-10 were found in individuals carrying the rs11642873/AA genotype. In conclusion, this study indicates that IRF8 may contribute to the genetic susceptibility of BD by regulating IRF8 expression and cytokine production.

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Figures

Figure 1
Figure 1. The influence of rs17445836 and rs11642873 on the relative expression of IRF8.
The expression of IRF8 in PBMCs treated with anti-CD3/28 antibodies. PBMCs were obtained from healthy individuals with diverse genotypes of rs17445836 (a) and rs11642873 (b). Data show the mean ± SD. Pc: Bonferroni corrected p value, multiplied by 3.
Figure 2
Figure 2. The influence of rs17445836 genotypes on cytokine production.
The production of IFN-γ (a), IL-10 (b), IL-17 (c) in PBMCs obtained from healthy genotype controls. PBMCs were treated with anti-CD3/28 antibodies. Data show the mean ± SD. Pc: Bonferroni corrected p value, multiplied by 3.
Figure 3
Figure 3. The influence of rs11642873 genotypes on cytokine production.
The production of IFN-γ (a), IL-10 (b), IL-17 (c) in PBMCs treated with anti-CD3/28 antibodies. PBMCs were obtained from healthy genotyped controls. Data show the mean ± SD. Pc: Bonferroni corrected p value, multiplied by 3.
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