Comparative Study

. 2016 Jan 21:16:10.

doi: 10.1186/s12880-015-0078-8.

A comparison of the quality of image acquisition between the incident dark field and sidestream dark field video-microscopes

Edward Gilbert-Kawai¹, Jonny Coppel², Vassiliki Bountziouka³, Can Ince⁴, Daniel Martin^{5

6}; Caudwell Xtreme Everest and Xtreme Everest 2 Research Groups

Collaborators, Affiliations

Collaborators

Caudwell Xtreme Everest and Xtreme Everest 2 Research Groups:
V Ahuja, G Aref-Adib, R Burnham, A Chisholm, K Clarke, D Coates, M Coates, D Cook, M Cox, S Dhillon, C Dougall, P Doyle, P Duncan, M Edsell, L Edwards, L Evans, P Gardiner, M Grocott, P Gunning, N Hart, J Harrington, J Harvey, C Holloway, D Howard, D Hurlbut, C Imray, C Ince, M Jonas, J van der Kaaij, M Khosravi, N Kolfschoten, D Levett, H Luery, A Luks, D Martin, R McMorrow, P Meale, K Mitchell, H Montgomery, G Morgan, J Morgan, A Murray, M Mythen, S Newman, M O'Dwyer, J Pate, T Plant, M Pun, P Richards, A Richardson, G Rodway, J Simpson, C Stroud, M Stroud, J Stygal, B Symons, P Szawarski, A Van Tulleken, C Van Tulleken, A Vercueil, L Wandrag, M Wilson, J Windsor, B Basnyat, C Clarke, T Hornbein, J Milledge, J West, S Abraham, T Adams, W Anseeuw, R Astin, B Basnyat, O Burdall, J Carroll, A Cobb, J Coppel, O Couppis, J Court, A Cumptsey, T Davies, S Dhillon, N Diamond, C Dougall, T Geliot, E Gilbert-Kawai, G Gilbert-Kawai, E Gnaiger, M Grocott, C Haldane, P Hennis, J Horscroft, D Howard, S Jack, B Jarvis, W Jenner, G Jones, J van der Kaaij, J Kenth, A Kotwica, R B C Kumar, J Lacey, V Laner, D Levett, D Martin, P Meale, K Mitchell, Z Mahomed, J Moonie, A Murray, M Mythen, P Mythen, K O'Brien, I Ruggles-Brice, K Salmon, A Sheperdigian, T Smedley, B Symons, C Tomlinson, A Vercueil, L Wandrag, S Ward, A Wight, C Wilkinson, S Wythe, M Feelisch, E Gilbert-Kawai, M Grocott, M Hanson, D Levett, D Martin, K Mitchell, H Montgomery, R Moon, A Murray, M Mythen, M Peters

Affiliations

¹ University College London Centre for Altitude Space and Extreme Environment Medicine, UCLH NIHR Biomedical Research Centre, Institute of Sport and Exercise Health, 170 Tottenham Court Road, London, W1T 7HA, UK. e.gilbert@ucl.ac.uk.
² University College London Centre for Altitude Space and Extreme Environment Medicine, UCLH NIHR Biomedical Research Centre, Institute of Sport and Exercise Health, 170 Tottenham Court Road, London, W1T 7HA, UK. jonny.coppel@gmail.com.
³ Statistical Support Service, Population, Policy and Practice Programme, Institute of Child Health, University College London, London, England. v.bountziouka@ucl.ac.uk.
⁴ Department of Intensive Care, Erasmus MC University Hospital Rotterdam, 3000, Rotterdam, The Netherlands. c.ince@amc.uva.nl.
⁵ University College London Centre for Altitude Space and Extreme Environment Medicine, UCLH NIHR Biomedical Research Centre, Institute of Sport and Exercise Health, 170 Tottenham Court Road, London, W1T 7HA, UK. daniel.martin@ucl.ac.uk.
⁶ Division of Neonatology, Erasmus MC-Sophia Children's Hospital, Wytemaweg 80, P.O. Box 2060, 3000 CB, Rotterdam, Netherlands. daniel.martin@ucl.ac.uk.

PMID: 26797680
PMCID: PMC4722634
DOI: 10.1186/s12880-015-0078-8

Comparative Study

A comparison of the quality of image acquisition between the incident dark field and sidestream dark field video-microscopes

Edward Gilbert-Kawai et al. BMC Med Imaging. 2016.

. 2016 Jan 21:16:10.

doi: 10.1186/s12880-015-0078-8.

Authors

Edward Gilbert-Kawai¹, Jonny Coppel², Vassiliki Bountziouka³, Can Ince⁴, Daniel Martin^{5

6}; Caudwell Xtreme Everest and Xtreme Everest 2 Research Groups

Collaborators

Caudwell Xtreme Everest and Xtreme Everest 2 Research Groups:
V Ahuja, G Aref-Adib, R Burnham, A Chisholm, K Clarke, D Coates, M Coates, D Cook, M Cox, S Dhillon, C Dougall, P Doyle, P Duncan, M Edsell, L Edwards, L Evans, P Gardiner, M Grocott, P Gunning, N Hart, J Harrington, J Harvey, C Holloway, D Howard, D Hurlbut, C Imray, C Ince, M Jonas, J van der Kaaij, M Khosravi, N Kolfschoten, D Levett, H Luery, A Luks, D Martin, R McMorrow, P Meale, K Mitchell, H Montgomery, G Morgan, J Morgan, A Murray, M Mythen, S Newman, M O'Dwyer, J Pate, T Plant, M Pun, P Richards, A Richardson, G Rodway, J Simpson, C Stroud, M Stroud, J Stygal, B Symons, P Szawarski, A Van Tulleken, C Van Tulleken, A Vercueil, L Wandrag, M Wilson, J Windsor, B Basnyat, C Clarke, T Hornbein, J Milledge, J West, S Abraham, T Adams, W Anseeuw, R Astin, B Basnyat, O Burdall, J Carroll, A Cobb, J Coppel, O Couppis, J Court, A Cumptsey, T Davies, S Dhillon, N Diamond, C Dougall, T Geliot, E Gilbert-Kawai, G Gilbert-Kawai, E Gnaiger, M Grocott, C Haldane, P Hennis, J Horscroft, D Howard, S Jack, B Jarvis, W Jenner, G Jones, J van der Kaaij, J Kenth, A Kotwica, R B C Kumar, J Lacey, V Laner, D Levett, D Martin, P Meale, K Mitchell, Z Mahomed, J Moonie, A Murray, M Mythen, P Mythen, K O'Brien, I Ruggles-Brice, K Salmon, A Sheperdigian, T Smedley, B Symons, C Tomlinson, A Vercueil, L Wandrag, S Ward, A Wight, C Wilkinson, S Wythe, M Feelisch, E Gilbert-Kawai, M Grocott, M Hanson, D Levett, D Martin, K Mitchell, H Montgomery, R Moon, A Murray, M Mythen, M Peters

Affiliations

¹ University College London Centre for Altitude Space and Extreme Environment Medicine, UCLH NIHR Biomedical Research Centre, Institute of Sport and Exercise Health, 170 Tottenham Court Road, London, W1T 7HA, UK. e.gilbert@ucl.ac.uk.
² University College London Centre for Altitude Space and Extreme Environment Medicine, UCLH NIHR Biomedical Research Centre, Institute of Sport and Exercise Health, 170 Tottenham Court Road, London, W1T 7HA, UK. jonny.coppel@gmail.com.
³ Statistical Support Service, Population, Policy and Practice Programme, Institute of Child Health, University College London, London, England. v.bountziouka@ucl.ac.uk.
⁴ Department of Intensive Care, Erasmus MC University Hospital Rotterdam, 3000, Rotterdam, The Netherlands. c.ince@amc.uva.nl.
⁵ University College London Centre for Altitude Space and Extreme Environment Medicine, UCLH NIHR Biomedical Research Centre, Institute of Sport and Exercise Health, 170 Tottenham Court Road, London, W1T 7HA, UK. daniel.martin@ucl.ac.uk.
⁶ Division of Neonatology, Erasmus MC-Sophia Children's Hospital, Wytemaweg 80, P.O. Box 2060, 3000 CB, Rotterdam, Netherlands. daniel.martin@ucl.ac.uk.

PMID: 26797680
PMCID: PMC4722634
DOI: 10.1186/s12880-015-0078-8

Abstract

Background: The 'Cytocam' is a third generation video-microscope, which enables real time visualisation of the in vivo microcirculation. Based upon the principle of incident dark field (IDF) illumination, this hand held computer-controlled device was designed to address the technical limitations of its predecessors, orthogonal polarization spectroscopy and sidestream dark field (SDF) imaging. In this manuscript, we aimed to compare the quality of sublingual microcirculatory image acquisition between the IDF and SDF devices.

Methods: Using the microcirculatory image quality scoring (MIQS) system, (six categories scored as either 0 = optimal, 1 = acceptable, or 10 = unacceptable), two independent raters compared 30 films acquired using the Cytocam IDF video-microscope, to an equal number obtained with an SDF device. Blinded to the origin of the films, the raters were therefore able to score between 0 and 60 for each film analysed. The scores' distributions between the two techniques were compared.

Results: The median MIQS (95% CI) given to the SDF camera was 7 (1.5-12), as compared to 1 (0.5-1.0) for the IDF device (p < 0.0001). Of the six categories assessed by the MIQS, nearly one fifth of the SDF videos were scored as unacceptable for pressure (20%), content (20%), and stability (17%), with focus scoring deficiently 13% of the time. High agreement between the two raters scoring values was evident, with an intra-class correlation coefficient (ICC) of 0.96 (95% CI: 0.94, 0.98).

Conclusions: These results demonstrate that the quality of sublingual microcirculatory image acquisition is superior in the Cytocam IDF video-microscope, as compared to the SDF video-microscope.

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Figures

**Fig. 1**
Distribution plot of total score values given by both raters with mean (95 % CI)

**Fig. 2**
Bland Altman plot for the agreement between two raters

**Fig. 3**
Distribution of scores according to the technique used to acquire the image with median (95 % CI)

See this image and copyright information in PMC

References

1. Aykut G IY, Ince C.: A new generation computer controlled imaging sensor based hand held microscope for quantifying bedside microcirculatory alterations. In Annual update in Intensive Care and Emergency Medicine 2014 Edited by Vincent JL. Springer; 2014:pp. 367-pp. 385.
1. Groner W, Winkelman JW, Harris AG, Ince C, Bouma GJ, Messmer K, Nadeau RG. Orthogonal polarization spectral imaging: a new method for study of the microcirculation. Nat Med. 1999;5:1209–1212. doi: 10.1038/13529. - DOI - PubMed
1. Goedhart PT, Khalilzada M, Bezemer R, Merza J, Ince C. Sidestream Dark Field (SDF) imaging: a novel stroboscopic LED ring-based imaging modality for clinical assessment of the microcirculation. Opt Express. 2007;15:15101–15114. doi: 10.1364/OE.15.015101. - DOI - PubMed
1. Mik EG, Johannes T, Fries M. Clinical microvascular monitoring: a bright future without a future? Crit Care Med. 2009;37:2980–2981. doi: 10.1097/CCM.0b013e3181aff81c. - DOI - PubMed
1. Sherman H, Klausner S, Cook WA. Incident dark-field illumination: a new method for microcirculatory study. Angiology. 1971;22:295–303. doi: 10.1177/000331977102200507. - DOI - PubMed

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A comparison of the quality of image acquisition between the incident dark field and sidestream dark field video-microscopes

Collaborators

Affiliations

A comparison of the quality of image acquisition between the incident dark field and sidestream dark field video-microscopes

Authors

Collaborators

Affiliations

Abstract

Figures

References

Publication types

MeSH terms

LinkOut - more resources

Full Text Sources

Other Literature Sources

Medical