Correction: A Novel Role for the TIR Domain in Association with Pathogen-Derived Elicitors

Abstract

[This corrects the article DOI: 10.1371/journal.pbio.0050068.].

Fig 4. N Co-Immunoprecipitates with p50-U1, but Not with p50-U1-Ob

Fig 4. N associates with p50-U1. Total protein extracts from N. benthamiana leaves transiently coexpressing gN-TAP (top panel, lanes 1–3) and Cerulean alone (middle panel, lane 1), p50-U1-Cerulean (middle panel, lane 2), or p50-U1-Ob-Cerulean (middle panel, lane 3) were pulled down using anti-GFP antibodies. gN-TAP was present in a complex with p50-U1-Cerulean (bottom panel, lane 2), but not with Cerulean (bottom panel, lane 1) or p50-U1-Ob-Cerulean (bottom panel, lane 3).

Fig 6. p50-U1 Fails to Associate with N TIR Mutants

(A) N and deletion mutants used to determine the domain required for association with p50. Numbers in parentheses are deleted amino acid residues. Not drawn to scale. (B) gN-TAP and gN-mutant-TAP proteins were tested for their association with p50-U1-Cerulean by coimmunoprecipitation. Input of N and its mutants are shown in top panel and Cerulean or Cerulean-tagged proteins are shown in the middle panel. Bottom panel shows coimmunoprecipitation results. gN-TAP + Cerulean (lane 1); gN TAP + p50-U1-Cerulean (lane 2); NΔTIR-TAP + p50-U1-Cerulean (lane 3); NΔP-loop-TAP + p50-U1-Cerulean (lane 4); NΔNB-TAP + p50-U1-Cerulean (lane 5); NΔLRR2–14-TAP + p50-U1-Cerulean (lane 6); N(D46H)TAP + p50-U1-Cerulean (lane 7); N(W141S)TAP + p50-U1-Cerulean (lane 8); and gN-TAP + p50-U1-Ob-Cerulean (lane 9). NΔTIR-TAP and N-TIR point mutants do not coimmunoprecipitate with p50-U1-Cerulean. Lane M is the size marker, and protein size is in kDa.

Fig 8. N's TIR Domain Is Sufficient for Association with p50-U1

(A) Co-immunoprecipitation of gN-TIR-TAP and p50-U1-Cerulean. N’s TIR domain was expressed under the control of N’s endogenous 5’ and 3’ regulatory regions. Extracts from tissue co-expressing N(TIR)-TAP (top panel, lanes 1 and 2) and p50-U1-Cerulean (middle panel, lane 1) or p50-U1-Ob-Cerulean (middle panel, lane 2) were pulled down with anti-GFP antibodies. N(TIR)-TAP was pulled down with p50-U1-Cerulean (bottom panel, lane 1), but not with p50-U1-Ob-Cerulean (bottom panel, lane 2). Lane M is the size marker, and protein sizes are shown in kDa. (B) BiFC between N(TIR)-YN and p50-U1-YC. N(TIR)-YN exhibits BiFC with p50-U1-YC (column 1), but not with p50-U1-Ob-YC (column 2). The TIR domains of two related R proteins, BS4 and RPP5, were tested for their ability to associate in vivo with p50-U1-YC. BS4(TIR)-YC and RPP5(TIR)-YC were coexpressed with p50-U1-YC, but were unable to exhibit BiFC (columns 3 and 4, respectively). Scale bar represents 20 μm.